Oral Bioengineering and Regenerative Medicine Lab, Shanghai Key Laboratory of Stomatology, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, China.
Center of Craniofacial Orthodontics, Department of Oral and Maxillofacial Surgery, Ninth People's Hospital Affiliated to Shanghai Jiao Tong University, School of Medicine, 639 Zhizaoju Road, Shanghai, 200011, China.
Sci Rep. 2017 Jul 11;7(1):5077. doi: 10.1038/s41598-017-05392-z.
Typically, bone regenerative medicine is applied to repair bone defects in patients with osteoporosis. Meanwhile, there is an urgent need to develop safe and cheap drugs that induce bone formation. Icariin, which is reported to promote the osteogenesis of stem cells in vitro, is the main active component of Herba Epimedii. However, whether icariin could repair bone defects caused by osteoporosis remains unknown. In this study, an osteoporosis model in rats was established by an ovariectomy first, and then, the osteogenic and angiogenic differentiation of bone mesenchymal stem cells (BMSCs) treated with icariin was evaluated. Furthermore, calcium phosphate cement (CPC) scaffolds loaded with icariin were constructed and then implanted into nude mice to determine the optimal construction. To evaluate its osteogenic and angiogenic ability in vivo, this construction was applied to calvarial defect of the ovariectomized (OVX) rats accompanied with an icariin gavage. This demonstrated that icariin could up-regulate the expression of osteogenic and angiogenic genes in BMSCs. Meanwhile, osteoclast formation was inhibited. Moreover, CPC could act as a suitable icariin delivery system for repairing bone defects by enhancing osteogenesis and angiogenesis, while the systemic administration of icariin has an antiosteoporotic effect that promotes bone defect repair.
通常，骨再生医学被应用于修复骨质疏松症患者的骨缺损。同时，迫切需要开发安全且廉价的能诱导骨形成的药物。淫羊藿苷是淫羊藿的主要活性成分，据报道其能促进干细胞的成骨作用。然而，淫羊藿苷是否能修复由骨质疏松症引起的骨缺损尚不清楚。在这项研究中，首先通过卵巢切除术建立大鼠骨质疏松症模型，然后评估淫羊藿苷处理的骨髓间充质干细胞（BMSCs）的成骨和成血管分化。此外，构建了负载淫羊藿苷的磷酸钙水泥（CPC）支架，并将其植入裸鼠体内以确定最佳构建方案。为了评估其体内的成骨和成血管能力，将该构建物应用于卵巢切除术（OVX）大鼠的颅骨缺损，并进行淫羊藿苷灌胃。结果表明，淫羊藿苷可以上调 BMSCs 中成骨和成血管基因的表达。同时，抑制破骨细胞的形成。此外，CPC 可以作为一种合适的淫羊藿苷递送系统，通过增强成骨和血管生成来修复骨缺损，而淫羊藿苷的全身给药具有抗骨质疏松作用，能促进骨缺损修复。
