临床分离新生儿肠外和肠源肺炎克雷伯菌同源性分析。
Homology analysis between clinically isolated extraintestinal and enteral Klebsiella pneumoniae among neonates.
机构信息
Department of Laboratory Medicine, The Second Xiangya Hospital, Central South University, 139 Renmin Road, Changsha, 410011, Hunan, China.
Department of Laboratory Medicine, Jiangxi Cancer Hospital, Nanchang, Jiangxi, China.
出版信息
BMC Microbiol. 2021 Jan 11;21(1):25. doi: 10.1186/s12866-020-02073-2.
BACKGROUND
Klebsiella pneumoniae is a leading cause of hospital-associated (HA) infections. It has been reported that gastrointestinal colonization (GI) is likely to be a common and significant reservoir for the transmission and infections of K. pneumoniae in both adults and neonates. However, the homologous relationship between clinically isolated extraintestinal and enteral K. pneumoniae in neonates hasn't been characterized yet.
RESULTS
Forty-three isolates from 21 neonatal patients were collected in this study. The proportion of carbapenem resistance was 62.8%. There were 12 patients (12/21, 57.4%) whose antibiotic resistance phenotypes, genotypes, and ST types (STs) were concordant. Six sequence types were detected using MLST, with ST37 and ST54 being the dominant types. The results of MLST were consist with the results of PFGE.
CONCLUSIONS
These data showed that there might be a close homologous relationship between extraintestinal K. pneumoniae (EXKP) and enteral K. pneumoniae (EKP) in neonates, indicating that the K. pneumoniae from the GI tract is possibly to be a significant reservoir for causing extraintestinal infections.
背景
肺炎克雷伯菌是医院获得性（HA）感染的主要原因。据报道，胃肠道定植（GI）很可能是成人和新生儿中肺炎克雷伯菌传播和感染的常见且重要的储库。然而，尚未对新生儿临床分离的肠外和肠内肺炎克雷伯菌之间的同源关系进行表征。
结果
本研究共收集了 21 例新生儿患者的 43 株分离株。碳青霉烯类耐药的比例为 62.8%。有 12 名患者（12/21，57.4%）的抗生素耐药表型、基因型和 ST 型（STs）一致。使用 MLST 检测到 6 种序列类型，ST37 和 ST54 是主要类型。MLST 的结果与 PFGE 的结果一致。
结论
这些数据表明，新生儿的肠外肺炎克雷伯菌（EXKP）和肠内肺炎克雷伯菌（EKP）之间可能存在密切的同源关系，表明来自胃肠道的肺炎克雷伯菌可能是引起肠外感染的重要储库。
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