Menéndez Esther, Flores-Félix José David, Mulas Rebeca, Andrés Fernando González, Fernández-Pascual Mercedes, Peix Alvaro, Velázquez Encarna
Departamento de Microbiología y Genética, Universidad de Salamanca and Instituto Hispanoluso de Investigaciones Agrarias (CIALE), Salamanca, Spain.
Instituto de Medio Ambiente, Recursos Naturales y Biodiversidad (IRENA). Universidad de León, León, Spain.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2312-2316. doi: 10.1099/ijsem.0.001949. Epub 2017 Jul 12.
A bacterial strain designated RTAE36T was isolated from wheat roots in northern Spain. Phylogenetic analyses based on 16S rRNA gene sequence placed the isolate into the genus Paenibacillus with its closest relative being Paenibacillus borealis DSM 13188T with 97.7 % sequence similarity. Cells of the isolate were facultatively anaerobic, Gram-stain-positive, motile and sporulating rods. Catalase and oxidase were positive. Gelatin, casein and starch were not hydrolysed. Growth was supported by many carbohydrates and organic acids as carbon sources. MK-7 was the only menaquinone detected, and anteiso-C15 : 0, C16 : 0, iso-C14 : 0 and iso-C16 : 0 were the major fatty acids. The polar lipids profile consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, one unidentified aminolipid, two unidentified phospholipids, three unidentified phosphoaminolipids, one unidentified glycolipid and one unidentified lipid. meso-Diaminopimelic acid was detected in the cell-wall peptidoglycan. Strains RTAE36T and P. borealis DSM 13188T had an mean DNA-DNA relatedness of 39 % and differed in several phenotypic and chemotaxonomic characteristics, confirming that strain RTAE36T should be considered as a representative of a novel species of the genus Paenibacillus, for which the name Paenibacillus tritici sp. nov. is proposed. The type strain is RTAE36T (=LMG 29502T=CECT 9125T).
从西班牙北部小麦根部分离出一株命名为RTAE36T的细菌菌株。基于16S rRNA基因序列的系统发育分析将该分离株归入芽孢杆菌属，其最接近的亲缘种是北方芽孢杆菌DSM 13188T，序列相似性为97.7%。该分离株的细胞为兼性厌氧、革兰氏染色阳性、运动且产芽孢的杆菌。过氧化氢酶和氧化酶呈阳性。不水解明胶、酪蛋白和淀粉。许多碳水化合物和有机酸作为碳源可支持其生长。检测到的唯一甲基萘醌是MK-7，主要脂肪酸为anteiso-C15 : 0、C16 : 0、iso-C14 : 0和iso-C16 : 0。极性脂质谱由双磷脂酰甘油、磷脂酰甘油、磷脂酰乙醇胺、一种未鉴定的氨基脂质、两种未鉴定的磷脂、三种未鉴定的磷氨基脂质、一种未鉴定的糖脂和一种未鉴定的脂质组成。在细胞壁肽聚糖中检测到内消旋二氨基庚二酸。菌株RTAE36T和北方芽孢杆菌DSM 13188T的平均DNA-DNA相关性为39%，在几个表型和化学分类特征上存在差异，这证实菌株RTAE36T应被视为芽孢杆菌属一个新物种的代表，为此提出新名称小麦芽孢杆菌（Paenibacillus tritici sp. nov.）。模式菌株为RTAE36T（=LMG 29502T=CECT 9125T）。
