Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
Unidad Asociada Grupo de Interacción Planta-Microorganismo Universidad de Salamanca-IRNASA-CSIC.
Int J Syst Evol Microbiol. 2014 Sep;64(Pt 9):3028-3033. doi: 10.1099/ijs.0.060830-0. Epub 2014 Jun 13.
A bacterial strain designated RLAHU15(T) was isolated from root nodules of Lupinus albus in Spain. Phylogenetic analyses based on 16S rRNA gene sequences placed the isolate in the genus Paenibacillus, with its closest relatives being Paenibacillus catalpae D75(T), Paenibacillus glycanilyticus DS-1(T), Paenibacillus endophyticus PECAE04(T) and Paenibacillus xinjiangensis B538(T) with 98.8 %, 98.9 %, 97.4 % and 97.4 % similarity, respectively. DNA-DNA hybridization studies showed values lower than 45 % between the strain RLAHU15(T) and any of these species. The isolate was a Gram-stain positive, motile and sporulating rod. Catalase activity was weak and oxidase activity was positive. Casein and starch were hydrolysed but gelatin was not. Growth was supported by many carbohydrates and organic acids as carbon sources. MK-7 was the only menaquinone detected and anteiso-C15 : 0 and iso-C16 : 0 were the major fatty acids. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, three unidentified phospholipids and an unidentified lipid. meso-Diaminopimelic acid was detected in the peptidoglycan. The DNA G+C content was 54.4 mol%. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain RLAHU15(T) represents a novel species of the genus Paenibacillus, for which the name Paenibacillus lupini sp. nov. is proposed. The type strain is RLAHU15(T) ( = LMG 27296(T) = CECT 8235(T)).
一株名为 RLAHU15(T) 的细菌菌株从西班牙的白 Lupinus albus 根瘤中分离得到。基于 16S rRNA 基因序列的系统发育分析将该分离株归入 Paenibacillus 属，其最接近的亲缘种为 Paenibacillus catalpae D75(T)、Paenibacillus glycanilyticus DS-1(T)、Paenibacillus endophyticus PECAE04(T)和 Paenibacillus xinjiangensis B538(T)，相似度分别为 98.8%、98.9%、97.4%和 97.4%。DNA-DNA 杂交研究表明，RLAHU15(T)与这些种之间的数值低于 45%。该分离株为革兰氏阳性、运动和产孢的杆状菌。过氧化氢酶活性较弱，氧化酶活性阳性。水解酪蛋白和淀粉，但不水解明胶。许多碳水化合物和有机酸作为碳源支持生长。检测到的唯一menaquinone 为 MK-7，主要脂肪酸为 anteiso-C15:0 和 iso-C16:0。主要极性脂为双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、三种未鉴定的磷脂和一种未鉴定的脂。肽聚糖中检测到 meso-Diaminopimelic 酸。DNA G+C 含量为 54.4 mol%。系统发育、化学分类和表型分析表明，菌株 RLAHU15(T)代表 Paenibacillus 属的一个新种，现提议将其命名为 Lupinus lupini sp. nov.。模式菌株为 RLAHU15(T)（=LMG 27296(T)=CECT 8235(T)）。
