Departamento de Microbiología y Genética, Universidad de Salamanca, Salamanca, Spain.
Unidad Asociada Grupo de Interacción Planta-Microorganismo, Universidad de Salamanca-IRNASA-CSIC, Spain.
Int J Syst Evol Microbiol. 2013 Dec;63(Pt 12):4433-4438. doi: 10.1099/ijs.0.050310-0. Epub 2013 Jul 12.
A bacterial strain, designated PECAE04(T), was isolated from root nodules of Cicer arietinum in Spain. Phylogenetic analysis based on 16S rRNA gene sequence placed the isolate into the genus Paenibacillus with its closest relative being Paenibacillus castaneae Ch-32(T) with 98.4 % 16S rRNA gene sequence similarity followed by Paenibacillus glycanilyticus DS-1(T), Paenibacillus prosopidis PW21(T), Paenibacillus xinjiangensis B538(T) and Paenibacillus catalpae D75(T) with similarities ranging from 97.9 to 96.8 %. DNA-DNA hybridization measurements showed values lower than 20 % between the strain PECAE04(T) and any of these species. The isolate was a Gram-stain-positive, motile, sporulating rod. Catalase and oxidase activities were positive. Aesculin was hydrolysed but casein and gelatin were not. Acetoin production, H2S production, nitrate reduction and urease and caseinase production were negative. Growth was supported by many carbohydrates and organic acids as carbon sources. MK-7 was the predominant menaquinone and anteiso-C15 : 0, iso-C16 : 0 and C16 : 0 were the major fatty acids. Major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, a glycolipid, three phospholipids and an unidentified lipid. Meso-diaminopimelic acid was not detected in the peptidoglycan. The DNA G+C content was 52.9 mol%. Phylogenetic, chemotaxonomic and phenotypic analyses showed that strain PECAE04(T) should be considered to be a representative of a novel species of the genus Paenibacillus, for which the name Paenibacillus endophyticus sp. nov. is proposed. The type strain is PECAE04(T) ( = LMG 27297(T) = CECT 8234(T)).
一株细菌菌株，PECAE04(T)，从西班牙的鹰嘴豆根瘤中分离得到。基于 16S rRNA 基因序列的系统发育分析将该分离株归入芽孢杆菌属，与最接近的亲缘关系是栗色糖多孢菌 Ch-32(T)，其 16S rRNA 基因序列相似性为 98.4%，其次是解淀粉芽孢杆菌 DS-1(T)、多粘类芽孢杆菌 PW21(T)、新疆芽孢杆菌 B538(T)和 Catalpae 芽孢杆菌 D75(T)，相似度在 97.9%到 96.8%之间。菌株 PECAE04(T)与这些种之间的 DNA-DNA 杂交测量值低于 20%。该分离株为革兰氏阳性、能动、产芽孢的杆状菌。过氧化氢酶和氧化酶活性均为阳性。七叶灵被水解，但明胶和明胶不被水解。丁酮酸产生、H2S 产生、硝酸盐还原和脲酶及明胶酶产生均为阴性。许多碳水化合物和有机酸作为碳源支持生长。MK-7 是主要的menaquinone，anteiso-C15:0、iso-C16:0 和 C16:0 是主要的脂肪酸。主要的极性脂是双磷脂酰甘油、磷脂酰乙醇胺、磷脂酰甘油、糖脂、三种磷脂和一种未知的脂。肽聚糖中未检测到 meso-二氨基庚二酸。DNA G+C 含量为 52.9 mol%。系统发育、化学分类和表型分析表明，菌株 PECAE04(T)应被视为芽孢杆菌属的一个新种的代表，建议将其命名为内生芽孢杆菌 Paenibacillus endophyticus sp. nov.。模式菌株为 PECAE04(T)（=LMG 27297(T)=CECT 8234(T)）。
