Li Yong, Fang Wei, Xie Shou-Jiang, Yang Xuqi, Wang Lai-Fa
The Key Laboratory of State Forestry Administration on Forest Protection, Research Institute of Forest Ecology Environment and Protection, Chinese Academy of Forestry, Beijing 100091, PR China.
Forestry of Bureau Taishan, Taishan 529200, PR China.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2248-2252. doi: 10.1099/ijsem.0.001934. Epub 2017 Jul 12.
A Gram-stain-positive, aerobic, non-motile, rod-shaped bacterial strain, designated 2C-7T, was isolated from symptomatic bark of a Populus × euramericana canker. Growth occurred between 10 and 37 °C and between pH 6 and 10, with optimal growth at 30 °C and pH 7.0-8.0. Growth was present under 0-8 % (w/v) salinity conditions (optimum 1-2 %). Growth occurred in the presence of 10 mM chromium (Cr6+). The major fatty acids (≥10 %) of the novel strain were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phospholipid, glycolipid and two unknown lipids. The major respiratory quinone was menaquinone MK-11. The cell wall amino acids were 2,4-diaminobutyric acid, alanine, glutamic acid and glycine. Strain 2C-7T was most similar to Leucobacter celer subsp. celer NAL101T (97.2 % 16S rRNA gene sequence similarity), 'Leucobacter kyeonggiensis' F3-P9T (97.1 %) and Leucobacter chromiireducens L-1T (97.1 %). In the phylogenetic tree, the isolate formed a single distinct branch separate from those of L. celer subsp. celer NAL101T, 'L. kyeonggiensis' F3-P9T and Leucobacter chironomi DSM 19883T. The DNA-DNA hybridization values between the novel strain and the reference strains were lower than the accepted bacterial threshold level of 70 % for species delineation. The DNA G+C content of strain 2C-7T was 70.0 mol%. Based on the data, strain 2C-7T represents a novel species in the genus Leucobacter, for which the name Leucobacter corticis sp. nov. is proposed. The type strain is 2C-7T (=CFCC 11901T=KCTC 39643T).
从美洲黑杨溃疡病有症状的树皮中分离出一株革兰氏染色阳性、需氧、无运动性、杆状的细菌菌株，命名为2C-7T。该菌株在10至37 °C以及pH值6至10的条件下生长，最适生长温度为30 °C，最适pH值为7.0 - 8.0。在0 - 8 %（w/v）盐度条件下（最适为1 - 2 %）能够生长。在10 mM铬（Cr6+）存在的情况下也能生长。该新菌株的主要脂肪酸（≥10 %）为anteiso-C15 : 0、anteiso-C17 : 0和iso-C16 : 0。极性脂类包括双磷脂酰甘油、磷脂酰甘油、磷脂、糖脂和两种未知脂类。主要呼吸醌为甲基萘醌MK-11。细胞壁氨基酸为2,4-二氨基丁酸、丙氨酸、谷氨酸和甘氨酸。菌株2C-7T与速生勒克氏菌勒克氏亚种NAL101T（16S rRNA基因序列相似性为97.2 %）、“京畿勒克氏菌”F3-P9T（97.1 %）和铬还原勒克氏菌L-1T（97.1 %）最为相似。在系统发育树中，该分离株形成了一个与速生勒克氏菌勒克氏亚种NAL101T、“京畿勒克氏菌”F3-P9T和嗜摇蚊勒克氏菌DSM 19883T不同的独立分支。该新菌株与参考菌株之间的DNA-DNA杂交值低于用于物种划分的公认细菌阈值70 %。菌株2C-7T的DNA G+C含量为70.0 mol%。基于这些数据，菌株2C-7T代表勒克氏菌属中的一个新物种，为此提出名为树皮勒克氏菌（Leucobacter corticis sp. nov.）。模式菌株为2C-7T（=CFCC 11901T=KCTC 39643T）。
