Lai Wei-An, Lin Shih-Yao, Hameed Asif, Hsu Yi-Han, Liu You-Cheng, Huang Hsuan-Ru, Shen Fo-Ting, Young Chiu-Chung
Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan ROC.
Department of Soil and Environmental Sciences, College of Agriculture and Natural Resources, National Chung Hsing University, Taiwan ROC.
Int J Syst Evol Microbiol. 2015 Dec;65(12):4734-4742. doi: 10.1099/ijsem.0.000640.
A novel yellow-pigmented, aerobic, rod-shaped, non-motile bacterium, designated strain CCMF41T, was isolated from rhizosphere soil of maize (Zea mays) collected in Wufeng District, Taichung, Taiwan. Strain CC-MF41T exhibited 16S rRNA gene sequence similarity of 97.5, 97.3, 97.2 and 97.1% to Leucobacter chironomi MM2LBT (and ‘Leucobacter kyeonggiensis’F3-P9 and ‘L. humi’ Re-6, the names of which have not been validly published), Leucobactertardus K70/01T, L. komagatae IFO 15245T and ‘Leucobacter margaritiformis’ A23. However,CC-MF41T and ‘L. margaritiformis’ A23 formed a loosely bound phylogenetic lineage (with alow bootstrap value) associated with species of the genus Leucobacter. In DNA–DNA reassociation experiments, the relatedness of strain CC-MF41T to L. chironomi DSM 19883T was 57.1% (reciprocal value 29.1 %). The DNA G+C content of strain CC-MF41T was 72.1 mol% and the cell-wall peptidoglycan contained 2,4-diaminobutyric acid, alanine, glycine,glutamic acid and threonine. The major menaquinone was MK-11 and the predominant fatty acids were iso-C16 : 0, anteiso-C15 : 0 and anteiso-C17 : 0. The polar lipid profile of strain CCMF41T contained major amounts of diphosphatidylglycerol followed by an unidentified glycolipid, phosphatidylglycerol and an unknown phospholipid. Based on its phylogenetic,phenotypic and chemotaxonomic distinctiveness, strain CC-MF41T represents a novel species of Leucobacter, for which the name Leucobacter zeae sp. nov. is proposed. The type strain isCC-MF41T (=BCRC 80515T=LMG 27265T).
从台湾台中市雾峰区采集的玉米（Zea mays）根际土壤中分离出一株新型黄色色素、需氧、杆状、无运动性的细菌，命名为CCMF41T菌株。CC-MF41T菌株与嗜摇蚊勒克菌MM2LBT（以及‘京畿勒克菌’F3-P9和‘土壤勒克菌’Re-6，其名称尚未有效发表）、迟缓勒克菌K70/01T、小川勒克菌IFO 15245T和‘珍珠状勒克菌’A23的16S rRNA基因序列相似性分别为97.5%、97.3%、97.2%和97.1%。然而，CC-MF41T和‘珍珠状勒克菌’A23形成了一个与勒克菌属物种相关的松散结合的系统发育谱系（自展值较低）。在DNA-DNA杂交实验中，CC-MF41T菌株与嗜摇蚊勒克菌DSM 19883T的相关性为57.1%（ reciprocal值为29.1%）。CC-MF41T菌株的DNA G+C含量为72.1 mol%，细胞壁肽聚糖含有2,4-二氨基丁酸、丙氨酸、甘氨酸、谷氨酸和苏氨酸。主要甲基萘醌为MK-11，主要脂肪酸为异-C16 : 0、anteiso-C15 : 0和anteiso-C17 : 0。CC-MF41T菌株的极性脂质谱含有大量二磷脂酰甘油，其次是一种未鉴定的糖脂、磷脂酰甘油和一种未知的磷脂。基于其系统发育、表型和化学分类学特征，CC-MF41T菌株代表勒克菌属的一个新物种，为此提出新名称玉米勒克菌（Leucobacter zeae sp. nov.）。模式菌株为CC-MF41T（=BCRC 80515T=LMG 27265T）。
