Department of Biology and Department of Biomedical and Pharmaceutical Sciences, Kyung Hee University, Seoul, 02447, Republic of Korea.
J Microbiol. 2021 Apr;59(4):360-368. doi: 10.1007/s12275-021-0472-6. Epub 2021 Jan 26.
Three novel bacterial strains, HDW9A, HDW9B, and HDW9C, isolated from the intestine of the diving beetles Cybister lewisianus and Cybister brevis, were characterized as three novel species using a polyphasic approach. The isolates were Gram-staining-positive, strictly aerobic, non-motile, and rod-shaped. They grew optimally at 30°C (pH 7) in the presence of 0.5% (wt/vol) NaCl. Phylogenetic analysis based on the 16S rRNA gene sequences revealed that they belong to the genus Leucobacter and are closely related to L. denitrificans M1T8B10 (98.4-98.7% sequence similarity). Average nucleotide identity (ANI) values among the isolates were 76.4-84.1%. ANI values for the isolates and the closest taxonomic species, L. denitrificans KACC 14055, were 72.3-73.1%. The isolates showed ANI values of < 76.5% with all analyzable Leucobacter strains in the EzBioCloud database. The genomic DNA G + C content of the isolates was 60.3-62.5%. The polar lipid components were phosphatidylglycerol, diphosphatidylglycerol, and other unidentified glycolipids, phospholipids, and lipids. The major cellular fatty acids were anteiso-C, iso-C, and anteiso-C. MK-10 was the major respiratory quinone, and MK-7 and MK-11 were the minor respiratory quinones. The whole-cell sugar components of the isolates were ribose, glucose, galactose, and mannose. The isolates harbored L-2,4-diaminobutyric acid, L-serine, L-lysine, L-aspartic acid, glycine, and D-glutamic acid within the cell wall peptidoglycan. Based on phylogenetic, phenotypic, chemotaxonomic, and genotypic analyses, strains HDW9A, HDW9B, and HDW9C represent three novel species within the genus Leucobacter. We propose the name Leucobacter coleopterorum sp. nov. for strain HDW9A (= KACC 21331 = KCTC 49317 = JCM 33667), the name Leucobacter insecticola sp. nov. for strain HDW9B (= KACC 21332 = KCTC 49318 = JCM 33668), and the name Leucobacter viscericola sp. nov. for strain HDW9C (= KACC 21333 = KCTC 49319 = JCM 33669).
从潜水甲虫 Cybister lewisianus 和 Cybister brevis 的肠道中分离到的三株新型细菌菌株 HDW9A、HDW9B 和 HDW9C，通过多相研究被鉴定为三个新种。这些分离株革兰氏染色阳性，严格需氧，不运动，呈杆状。它们在 30°C（pH 7）、0.5%（wt/vol）NaCl 存在下最佳生长。基于 16S rRNA 基因序列的系统发育分析表明，它们属于 Leucobacter 属，与 L. denitrificans M1T8B10（98.4-98.7%序列相似性）密切相关。分离株之间的平均核苷酸同一性（ANI）值为 76.4-84.1%。分离株与最接近的分类种 L. denitrificans KACC 14055 的 ANI 值为 72.3-73.1%。分离株与 EzBioCloud 数据库中所有可分析的 Leucobacter 菌株的 ANI 值均<76.5%。分离株的基因组 DNA G+C 含量为 60.3-62.5%。极性脂成分是磷脂酰甘油、双磷脂酰甘油和其他未鉴定的糖脂、磷脂和脂类。主要的细胞脂肪酸是 anteiso-C、iso-C 和 anteiso-C。MK-10 是主要的呼吸醌，MK-7 和 MK-11 是次要的呼吸醌。分离株的全细胞糖组分为核糖、葡萄糖、半乳糖和甘露糖。分离株的细胞壁肽聚糖中含有 L-2,4-二氨基丁酸、L-丝氨酸、L-赖氨酸、L-天冬氨酸、甘氨酸和 D-谷氨酸。基于系统发育、表型、化学生态和基因型分析，菌株 HDW9A、HDW9B 和 HDW9C 代表 Leucobacter 属中的三个新种。我们提议将菌株 HDW9A（=KACC 21331=KCTC 49317=JCM 33667）命名为 Leucobacter coleopterorum sp. nov.，将菌株 HDW9B（=KACC 21332=KCTC 49318=JCM 33668）命名为 Leucobacter insecticola sp. nov.，将菌株 HDW9C（=KACC 21333=KCTC 49319=JCM 33669）命名为 Leucobacter viscericola sp. nov.。
