Benga Laurentiu, Spröer Cathrin, Schumann Peter, Verbarg Susanne, Bunk Boyke, Engelhardt Eva, Benten W Peter M, Sager Martin
1Central Unit for Animal Research and Animal Welfare Affairs, University Hospital, Heinrich - Heine - University, Geb. 22.22, Universitätsstr. 1, 40225 Düsseldorf, Germany.
2Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstraße 7B, 38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2019 Jul;69(7):2095-2100. doi: 10.1099/ijsem.0.003446. Epub 2019 May 17.
A Gram-stain-positive, rod-shaped, aerobic, non-motile, white, opaque bacterial isolate, designated 924/12, was isolated from the nose of a laboratory mouse in Düsseldorf, Germany. The 16S rRNA gene sequence analyses indicated the phylogenetic position of the strain within the genus Leucobacter. Similarity levels over 97 % were recorded between the 16S rRNA gene sequence of strain 924/12 and the type strains of the species Leucobacter chironomi DSM 19883 (99.5 %), followed by Leucobacter celersubsp. astrifaciens CBX151 (97.6 %), Leucobacter celersubsp. celer NAL101 (97.5 %), 'Leucobacter kyeonggiensis' F3-P9 (97.5 %), Leucobacter zeae CC-MF41 (97.3 %), Leucobacter chromiiresistens JG31 (97.1 %), Leucobacter triazinivorans JW-1 (97.1 %), Leucobacter corticis 2 C-7 (97.0 %) and Leucobacter aridicolis CIP108388 (97.0 %). DNA-DNA hybridization and whole genomic comparison, mandatory to taxonomically separate strain 924/12 from the type strain of L. chironomi, revealed similarity values of 40.4 and 30.8 %, respectively, thus below the threshold of 70 % recommended differentiating between species. The cell-wall amino acids of the novel isolate were diaminobutyric acid, alanine, glycine, threonine and glutamic acid. The major fatty acids were anteiso-C15 : 0, anteiso-C17 : 0 and iso-C16 : 0. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, glycolipid and one unknown lipid, whereas the predominant menaquinones were MK-11 and MK-10. The genomic DNA G+C content of strain 924/12 was 70.6 mol%. Phylogenetic analyses based on the 16S rRNA gene sequences and the phenotypical differences between strain 924/12 and the other closely related type strains of the genus Leucobacter indicated that strain 924/12 represents a novel species within the genus Leucobacter, family Microbacteriaceae, for which the name Leucobacter muris sp. nov. is proposed. The type strain is 924/12 (=DSM 101948=CCM 8761).
从德国杜塞尔多夫一只实验小鼠的鼻腔中分离出一株革兰氏染色阳性、杆状、需氧、不运动、白色、不透明的细菌菌株，编号为924/12。16S rRNA基因序列分析表明该菌株在勒克氏菌属中的系统发育位置。菌株924/12的16S rRNA基因序列与嗜摇蚊勒克氏菌DSM 19883模式菌株（99.5%）的相似性水平超过97%，其次是敏捷勒克氏菌星形亚种CBX151（97.6%）、敏捷勒克氏菌敏捷亚种NAL101（97.5%）、“京畿道勒克氏菌”F3 - P9（97.5%）、玉米勒克氏菌CC - MF41（97.3%）、耐铬勒克氏菌JG31（97.1%）、三嗪降解勒克氏菌JW - 1（97.1%）、皮质勒克氏菌2 C - 7（97.0%）和干旱勒克氏菌CIP108388（97.0%）。DNA - DNA杂交和全基因组比较是将菌株924/12与嗜摇蚊勒克氏菌模式菌株进行分类区分的必要手段，结果显示相似性值分别为40.4%和30.8%，低于推荐的区分物种的70%阈值。新分离菌株的细胞壁氨基酸为二氨基丁酸、丙氨酸、甘氨酸、苏氨酸和谷氨酸。主要脂肪酸为anteiso - C15 : 0、anteiso - C17 : 0和iso - C16 : 0。主要极性脂为双磷脂酰甘油、磷脂酰甘油、糖脂和一种未知脂质，而主要甲基萘醌为MK - 11和MK - 10。菌株924/12的基因组DNA G + C含量为70.6 mol%。基于16S rRNA基因序列的系统发育分析以及菌株924/12与勒克氏菌属其他密切相关模式菌株之间的表型差异表明，菌株924/12代表勒克氏菌属、微杆菌科中的一个新物种，为此提出名称为鼠勒克氏菌（Leucobacter muris sp. nov.）。模式菌株为924/12（=DSM 101948 = CCM 8761）。
