Breider Sven, Freese Heike M, Spröer Cathrin, Simon Meinhard, Overmann Jörg, Brinkhoff Thorsten
Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, Carl-von-Ossietzky-Str. 9-11, 26129 Oldenburg, Germany.
Leibniz Institute DSMZ - German Collection of Microorganisms and Cell Cultures, Inhoffenstr. 7B, 38124 Braunschweig, Germany.
Int J Syst Evol Microbiol. 2017 Jul;67(7):2153-2159. doi: 10.1099/ijsem.0.001879. Epub 2017 Jul 12.
Three heterotrophic, aerobic, brown-pigmented strains, designated P97T, P100 and P104, were isolated from a harbour in the southern North Sea. Phylogenetic analysis of 16S rRNA gene sequences revealed that the isolates are affiliated to the genus Phaeobacter. In silico DNA-DNA hybridization of the genome of strain P97T against those of existing type species indicated that P97T represents a novel species within the genus Phaeobacter, with Phaeobacter inhibens T5T as the closest described organism (29.6 % DNA-DNA relatedness) followed by P. gallaeciensis CIP 105210T (26.4 %). DNA-DNA hybridization demonstrated that the three new strains belong to the same species. The new isolates inhibited Pseudoalteromonas tunicata DSM 14096T, and were Gram-stain-negative, catalase- and oxidase-positive, chemo-organoheterotrophic and motile. Growth occurred at pH 6.5-9.5 (optimum 7.0-8.0) and at 4-30 °C (optimum 20-28 °C). The strains required NaCl for growth. The salinity range was 0.5-6.0 % (w/v) NaCl for P97T and P100, and 0.5-5.0 % for P104, lower than values described for Phaeobacter gallaeciensis and Phaeobacter inhibens. The optimum NaCl concentration for strains P97T and P104 was 2.0-4.0 %, and for P100 was 2.0-3.0 %. Fatty acids (>1 %) comprised 18 : 1ω7c, 16 : 0, 18 : 1 ω7c 11-methyl, 18 : 0, 12 :1, 18 : 2ω7c,12, 10 : 0 3-OH and 12 : 0 3-OH. Polar lipids were phosphatidylcholine, phosphatidylethanolamine, phosphatidylglycerol, an aminolipid, one unknown lipid and one additional unknown lipid in strain P97T. The major respiratory quinone was Q10. Based on phylogenetic and phenotypic differences, the strains represent a novel species in the genus Phaeobacter, for which the name Phaeobacter porticola sp. nov. is proposed. The type strain is P97T (=DSM 103148T=LMG 29594T).
从北海南部的一个港口分离出三株异养、需氧、产褐色色素的菌株，分别命名为P97T、P100和P104。对16S rRNA基因序列进行系统发育分析表明，这些分离株隶属于海杆菌属。将菌株P97T的基因组与现有模式种的基因组进行电子DNA-DNA杂交分析表明，P97T代表海杆菌属内的一个新物种，与已描述的最接近的菌株抑制海杆菌T5T的DNA-DNA相关性为29.6%，其次是加氏海杆菌CIP 105210T（26.4%）。DNA-DNA杂交表明这三株新菌株属于同一物种。这些新分离株对被膜假交替单胞菌DSM 14096T具有抑制作用，它们革兰氏染色阴性、过氧化氢酶和氧化酶阳性、化能有机异养且具运动性。在pH 6.5-9.5（最适pH 7.0-8.0）和4-30 °C（最适温度20-28 °C）条件下生长。这些菌株生长需要NaCl。P97T和P100的盐度范围为0.5-6.0%（w/v）NaCl，P104为0.5-5.0%，低于加氏海杆菌和抑制海杆菌所描述的值。菌株P97T和P104的最适NaCl浓度为2.0-4.0%，P100为2.0-3.0%。脂肪酸（>1%）包括18:1ω7c、16:0、18:1 ω7c 11-甲基、18:0、12:1、18:2ω7c,12、10:0 3-OH和12:0 3-OH。极性脂类包括磷脂酰胆碱、磷脂酰乙醇胺、磷脂酰甘油、一种氨基脂类、一种未知脂类以及菌株P97T中的另一种未知脂类。主要呼吸醌为Q10。基于系统发育和表型差异，这些菌株代表海杆菌属中的一个新物种，为此提出新名称港口海杆菌（Phaeobacter porticola sp. nov.）。模式菌株为P97T（=DSM 103148T=LMG 29594T）。
