Klotz Franziska, Brinkhoff Thorsten, Freese Heike M, Wietz Matthias, Teske Andreas, Simon Meinhard, Giebel Helge-Ansgar
Institute for Chemistry and Biology of the Marine Environment, University of Oldenburg, D-26111 Oldenburg, Germany.
Present address: Department of Biology, University of Konstanz, 78464 Konstanz, Germany.
Int J Syst Evol Microbiol. 2018 Mar;68(3):736-744. doi: 10.1099/ijsem.0.002573. Epub 2018 Feb 6.
A heterotrophic, Gram-stain-negative, aerobic, sodium-requiring and motile bacterium was isolated from oil-contaminated surface water of the Gulf of Mexico during the Deepwater Horizon oil spill. Strain O3.65 showed highest 16S rRNA gene sequence similarity to Phaeobacter gallaeciensis BS107 and Phaeobacter inhibens T5, both with 98.3 %, respectively. Based on complete genome analysis, highest similarity was observed to species of the genus Ruegeria. Strain O3.65 exhibited a broad salinity, temperature and pH range of 0.5-10 % NaCl, 4-45 °C and 5.5-9.0, respectively. The DNA G+C content of strain O3.65 was 61.5 mol%. The major respiratory lipoquinone was ubiquinone-10 (Q-10), the most dominant fatty acids (>1 %) comprised 18 : 1ω7c and 18 : 1ω7c 11-methyl, 10 : 0 3OH, 12 : 1 3OH, 14 : 1 3OH/3-oxo-14 : 0, 16 : 0, 16 : 0 2OH, 18 : 1 2OH and 12 : 1. The polar lipid pattern indicated presence of phosphatidylcholine, phosphatidylglycerol, an unidentified aminolipid, two unidentified phospholipids and seven unidentified lipids. On Difco marine broth agar, strain O3.65 formed smooth, shiny white to beige and convex colonies with regular edges. Phylogenetic, phylogenomic and phenotypic differences revealed that strain O3.65 represents a new species of a novel genus within the family Rhodobacteraceae, for which we propose the name Tritonibacter horizontis gen. nov., sp. nov. The type strain of the type species is O3.65 (=DSM 101689=LMG 29740).
在“深水地平线”漏油事件期间，从墨西哥湾受石油污染的地表水分离出一株异养、革兰氏染色阴性、需氧、嗜钠且具运动性的细菌。菌株O3.65的16S rRNA基因序列与加氏海杆菌BS107和抑制海杆菌T5的相似性最高，均为98.3%。基于全基因组分析，观察到与鲁杰氏菌属物种的相似性最高。菌株O3.65分别表现出0.5 - 10% NaCl、4 - 45°C和5.5 - 9.0的宽盐度、温度和pH范围。菌株O3.65的DNA G + C含量为61.5 mol%。主要呼吸型脂醌为泛醌-10（Q - 10），最主要的脂肪酸（>1%）包括18:1ω7c和18:1ω7c 11 - 甲基、10:0 3OH、12:1 3OH、14:1 3OH/3 - 氧代-14:0、16:0、16:0 2OH、18:1 2OH和12:1。极性脂质模式表明存在磷脂酰胆碱、磷脂酰甘油、一种未鉴定的氨基脂质、两种未鉴定的磷脂和七种未鉴定的脂质。在Difco海洋肉汤琼脂上，菌株O3.65形成边缘规则的光滑、有光泽的白色至米色凸形菌落。系统发育、系统基因组学和表型差异表明，菌株O3.65代表红杆菌科内一个新属的新物种，我们将其命名为水平海栖杆菌属（Tritonibacter），新种（horizontis）。模式种的模式菌株为O3.65（=DSM 101689 = LMG 29740）。
