Gan Lu, Liu Zheng, Wei Ming, Chen Yulong, Yang Xiaomei, Chen Lihong, Xiao Xiaomin
Department of Gynecology, Shaanxi Provincial People's Hospital, Xi'an, Shaanxi Department of Gynaecology and Obstetrics, the First Affiliated Hospital of Jinan University, Guangzhou Department of Pathology and Molecular Medicine, Xi'an Jiaotong University Health Science Center, Hanzhong Department of Pharmacology Shaanxi Key Laboratory of Ischemic Cardiovascular Disease, Institute of Basic and Translational Medicine, Xi'an Medical University, Xi'an, Shaanxi, China.
Medicine (Baltimore). 2017 Jul;96(28):e7515. doi: 10.1097/MD.0000000000007515.
Pre-eclampsia (PE) is one of the leading causes of maternal and neonatal morbidity and mortality. In recent years, many studies have shown that microRNAs (miRNA) play important roles in the development of PE. However, the molecular pathogenesis of PE remains unknown.In the present study, we performed a case-control study to verify the differential expression of 4 candidate miRNAs (miR-210, miR-155, miR-125b-5p, and miR-125a-5p) in 20 PE pregnancies and 20 healthy pregnancies. The real-time quantitative reverse transcriptase-polymerase chain reaction has been utilized to estimate the Ct values in both groups.Our results have shown that miR-210 and miR-155 were upregulated in serum of PE pregnancies, which suggest a potential association between these 2 miRNAs and the pathogenesis of PE. Further studies showed that the area under the receiver operating characteristic curve (AUC) of miR-210 and miR-155 were 0.750 and 0.703, respectively. The AUC of the expression ratio of miR-210 (serum/urine) and miR-155 (serum/urine) were 0.761 and 0.718, respectively. Moreover, 24-hour urine proteins have positive correlation with urine miR-210 and miR-155.Our findings indicated that serum miR-210 and miR-155 could be 2 sensitivity and specificity biomarkers for the diagnosis of PE while urine miR-210 and miR-155 both could be used to evaluate the severity of kidney injury. Using these miRNAs may provide a novel diagnosis method for identifying pregnant women who are at risk for developing PE.
子痫前期（PE）是孕产妇和新生儿发病及死亡的主要原因之一。近年来，许多研究表明，微小RNA（miRNA）在PE的发生发展中起重要作用。然而，PE的分子发病机制仍不清楚。在本研究中，我们进行了一项病例对照研究，以验证4种候选miRNA（miR-210、miR-155、miR-125b-5p和miR-125a-5p）在20例PE妊娠和20例健康妊娠中的差异表达。采用实时定量逆转录-聚合酶链反应来估计两组的Ct值。我们的结果表明，miR-210和miR-155在PE妊娠血清中上调，这表明这两种miRNA与PE的发病机制之间可能存在关联。进一步研究表明，miR-210和miR-155的受试者工作特征曲线下面积（AUC）分别为0.750和0.703。miR-210（血清/尿液）和miR-155（血清/尿液）表达比值的AUC分别为0.761和0.718。此外，24小时尿蛋白与尿miR-210和miR-155呈正相关。我们的研究结果表明，血清miR-210和miR-155可能是诊断PE的两个具有敏感性和特异性的生物标志物，而尿miR-210和miR-155均可用于评估肾损伤的严重程度。使用这些miRNA可能为识别有发生PE风险的孕妇提供一种新的诊断方法。
