Roux Véronique, Fenner Lukas, Raoult Didier
Laboratoire de Bactériologie-Virologie, Hôpital de la Timone, CNRS UMR 6020, IFR48, 264 rue Saint-Pierre, 13385 Marseille Cedex 05, France.
Int J Syst Evol Microbiol. 2008 Mar;58(Pt 3):682-7. doi: 10.1099/ijs.0.65228-0.
Gram-negative, spore-forming rods were isolated from a human urine sample (strain 5402403(T)) and a human cerebrospinal fluid sample (strain 4401170(T)). Based on genotypic characteristics, these strains belonged to the genus Paenibacillus and were closely related. Phylogenetic analysis based on 16S rRNA gene sequence comparison showed that they clustered with Paenibacillus massiliensis 2301065(T) (95.9 and 94.3 % 16S rRNA gene sequence similarity, respectively, for strains 5402403(T) and 4401170(T)), Paenibacillus illinoisensis NRRL NRS-61356(T) (90.6 and 93.8 %), Paenibacillus xylanilyticus XIL14(T) (95.3 and 95.4 %), Paenibacillus barcinonensis BP-23(T) (94.3 and 94.0 %), Paenibacillus pabuli NCIMB 12781(T) (89.1 and 92.3 %) and Paenibacillus amylolyticus NRRL NRS-290(T) (94.2 and 93.8 %). The predominant fatty acids were 15 : 0 anteiso (49.0 and 55.3 %, respectively, for strains 5402403(T) and 4401170(T)), 16 : 0 iso (15.4 and 13.5 %), 16 : 0 (7.6 and 3.6 %), 15 : 0 (6.3 and 2.8 %), 17 : 0 anteiso (5.7 and 7.5 %), 14 : 0 iso (4.1 and 2.7 %) and 15 : 0 iso (4.1 and 3.4 %). 16S rRNA gene sequence similarity between strain 4401170(T) and strain 5402403(T) was 98.4 %, but the DNA-DNA reassociation rate between the two strains was 53.2 %. So, considering the recommendations of the ad hoc committee, they do not belong to the same species. On the basis of phenotypic data and genotypic inference, it is proposed that the strains should be assigned to the novel species Paenibacillus urinalis sp. nov. (type strain 5402403(T) =CIP 109357(T) =CCUG 53521(T)) and Paenibacillus provencensis sp. nov. (type strain 4401170(T) =CIP 109358(T) =CCUG 53519(T)).
革兰氏阴性、产芽孢杆菌分别从一份人类尿液样本（菌株5402403(T)）和一份人类脑脊液样本（菌株4401170(T)）中分离得到。基于基因型特征，这些菌株属于芽孢杆菌属且亲缘关系密切。基于16S rRNA基因序列比较的系统发育分析表明，它们与马赛芽孢杆菌2301065(T)聚类（菌株5402403(T)和4401170(T)的16S rRNA基因序列相似性分别为95.9%和94.3%）、伊利诺伊芽孢杆菌NRRL NRS - 61356(T)（90.6%和93.8%）、木聚糖分解芽孢杆菌XIL14(T)（95.3%和95.4%）、巴塞罗那芽孢杆菌BP - 23(T)（94.3%和94.0%）、巴氏芽孢杆菌NCIMB 12781(T)（89.1%和92.3%）以及解淀粉芽孢杆菌NRRL NRS - 290(T)（94.2%和93.8%）。主要脂肪酸为15 : 0 anteiso（菌株5402403(T)和4401170(T)分别为49.0%和55.3%）、16 : 0 iso（15.4%和13.5%）、16 : 0（7.6%和3.6%）、15 : 0（6.3%和2.8%）、17 : 0 anteiso（5.7%和7.5%）、14 : 0 iso（4.1%和2.7%）以及15 : 0 iso（4.1%和3.4%）。菌株4401170(T)与菌株5402403(T)之间的16S rRNA基因序列相似性为98.4%，但两菌株间的DNA - DNA杂交率为53.2%。因此，根据特设委员会的建议，它们不属于同一物种。基于表型数据和基因型推断，建议将这些菌株归为新物种尿道芽孢杆菌（Paenibacillus urinalis sp. nov.）（模式菌株5402403(T) =CIP 109357(T) =CCUG 53521(T)）和普罗旺斯芽孢杆菌（Paenibacillus provencensis sp. nov.）（模式菌株4401170(T) =CIP 109358(T) =CCUG 53519(T)）。
