Ujah G A, Nna V U, Agah M I, Omue L O, Leku C B, Osim E E
Department of Physiology, Faculty of Basic Medical Sciences, College of Medical Sciences, University of Calabar, Calabar, Cross River State, Nigeria.
Department of Physiology, School of Medical Sciences, University Sains Malaysia, Kelantan, Malaysia.
Andrologia. 2018 Mar;50(2). doi: 10.1111/and.12866. Epub 2017 Jul 13.
Cadmium chloride (CdCl ) has been reported to cause reproductive toxicity in male rats, mainly through oxidative stress. This study examined its effect on sexual behaviour, as one of the mechanisms of reproductive dysfunction, as well as the possible ameliorative effect of quercetin (QE) on same. Thirty male Wistar rats (10 weeks old), weighing 270-300 g, were used for this study. They were either orally administered 2% DMSO, CdCl (5 mg/kg b.w.), QE (20 mg/kg b.w.) or CdCl +QE, once daily for 4 weeks, before sexual behavioural studies. The 5th group received CdCl for 4 weeks and allowed 4-week recovery period, before sexual behavioural test. Rats were sacrificed after sexual behavioural studies. The blood, testis and penis were collected for biochemical assays. Cadmium increased mount, intromission and ejaculatory latencies, but reduced their frequencies, compared to control. Serum nitric oxide increased, while penile cyclic guanosine monophosphate reduced in the CdCl -exposed rats, compared to control. CdCl increased testicular cholesterol, but reduced 3β-hydroxysteroid dehydrogenase (3β-HSD) and 17β-HSD activities, and testosterone concentration. QE better attenuated these negative changes compared to withdrawal of CdCl treatment. In conclusion, CdCl suppressed steroidogenesis, penile erection and sexual behaviour, with poor reversal following withdrawal, while QE attenuated these effects.
据报道，氯化镉（CdCl₂）会导致雄性大鼠生殖毒性，主要是通过氧化应激。本研究考察了其对性行为（作为生殖功能障碍的机制之一）的影响，以及槲皮素（QE）对其可能的改善作用。选用30只10周龄、体重270 - 300克的雄性Wistar大鼠进行本研究。在进行性行为研究前，它们被分别每日经口给予2%二甲亚砜、CdCl₂（5毫克/千克体重）、QE（20毫克/千克体重）或CdCl₂ + QE，持续4周。第5组接受CdCl₂处理4周，然后在性行为测试前给予4周恢复期。性行为研究后处死大鼠。采集血液、睾丸和阴茎用于生化分析。与对照组相比，镉增加了爬跨、插入和射精潜伏期，但降低了它们的频率。与对照组相比（下同），CdCl₂暴露大鼠血清一氧化氮增加，而阴茎环磷酸鸟苷减少。CdCl₂增加了睾丸胆固醇，但降低了3β - 羟基类固醇脱氢酶（3β - HSD）和17β - 羟基类固醇脱氢酶（17β - HSD）活性以及睾酮浓度。与停止CdCl₂处理相比，QE能更好地减轻这些负面变化。总之，CdCl₂抑制类固醇生成、阴茎勃起和性行为，停止处理后逆转效果不佳，而QE能减轻这些影响。
