Caussé Elizabeth, Fournier Pauline, Roncalli Jérome, Salvayre Robert, Galinier Michel
a Biochemistry Laboratory , Rangueil University Hospital , Toulouse , France.
b Department of Cardiology , Rangueil University Hospital , Toulouse , France.
Acta Cardiol. 2017 Aug;72(4):397-403. doi: 10.1080/00015385.2017.1335104. Epub 2017 Jul 14.
Background Oxidative stress (OS) represents the primary mediator of chronic heart failure (CHF) development and progression. It is well established that homocysteine is able to generate reactive oxygen species. Small amounts of allantoin in human serum result from free radical action on urate and may provide a stable marker for in vivo free radical activity. To investigate whether some easily measurable indexes such as antioxidants (uric acid, glutathione) and related molecules (allantoin, homocysteine and cysteine) can serve as OS biomarkers. Methods We investigated 75 stable CHF patients. Aminothiols and purine compound levels were determined by capillary electrophoresis. Results The homocysteine level was markedly elevated in CHF patients, whatever the aetiology. Parameters of the transsulfuration pathway and the investigated purine compounds were significantly increased. Conversely, total glutathione was decreased. The allantoin/uric acid ratio was significantly higher in CHF patients with an hyperhomocysteinaemia >17 μmol/L. All parameters of the transsulfuration and purine degadation pathways were significantly correlated, suggesting an OS in CHF patients. Conclusion Our data show an imbalance of serum aminothiols and purine compounds in these CHF patients on adapted therapy. We suggest that the evaluation and control of these new markers may help improve the OS that participates in the progression of the disease.
背景 氧化应激（OS）是慢性心力衰竭（CHF）发生和发展的主要介导因素。众所周知，同型半胱氨酸能够产生活性氧。人血清中的少量尿囊素是自由基作用于尿酸的结果，可能为体内自由基活性提供一个稳定的标志物。为了研究一些易于测量的指标，如抗氧化剂（尿酸、谷胱甘肽）和相关分子（尿囊素、同型半胱氨酸和半胱氨酸）是否可作为OS生物标志物。方法 我们对75例稳定期CHF患者进行了研究。通过毛细管电泳测定氨基硫醇和嘌呤化合物水平。结果 无论病因如何，CHF患者的同型半胱氨酸水平均显著升高。转硫途径参数和所研究的嘌呤化合物均显著增加。相反，总谷胱甘肽减少。同型半胱氨酸血症>17 μmol/L的CHF患者中，尿囊素/尿酸比值显著更高。转硫和嘌呤降解途径的所有参数均显著相关，提示CHF患者存在OS。结论 我们的数据显示，这些接受适应性治疗的CHF患者血清氨基硫醇和嘌呤化合物失衡。我们建议，对这些新标志物的评估和控制可能有助于改善参与疾病进展的OS。
