Kaluzhnaya O V, Itskovich V B
Limnological Institute, Siberian Branch, Russian Academy of Science, Irkutsk, 664030 Russia.
Mol Biol (Mosk). 2017 May-Jun;51(3):423-430. doi: 10.7868/S0026898417030089.
The psbA gene, which encodes a major photosystem II protein (protein II or D1), is a marker for the presence of phototrophic organisms in water communities. We have pioneered the use of this marker for studying the diversity of phototrophic microflora of freshwater invertebrates. The object of the study is the microbial associations accompanying the endemic Baikal sponge Baikalospongia intermedia and the surrounding aquatic microbial community. Analysis of the psbA gene sequences in the examined microbiomes demonstrates the presence of various phototrophic groups, such as Cyanobacteria, Chlorophyta, Heterokonta, Haptophyta, and Ochrophyta algae, as well as cyanophages. A total of 35 unique psbA gene sequences have been distinguished in the microbial communities of the endemic sponge B. intermedia and 32 unique sequences in the water community surrounding the sponge. These data demonstrate the involvement of sponge symbiotic communities in the accumulation of primary production and carbon cycle in the Lake Baikal ecosystem.
编码主要光系统II蛋白（蛋白II或D1）的psbA基因，是水群落中光合生物存在的一个标志物。我们率先使用这个标志物来研究淡水无脊椎动物光合微生物区系的多样性。本研究的对象是伴随着贝加尔湖特有海绵中间贝加尔海绵以及周围水生微生物群落的微生物组合。对所检测微生物群落中psbA基因序列的分析表明，存在各种光合类群，如蓝细菌、绿藻、不等鞭毛类、定鞭藻和褐藻门藻类，以及蓝噬菌体。在特有海绵中间贝加尔海绵的微生物群落中总共鉴别出35个独特的psbA基因序列，在海绵周围的水群落中有32个独特序列。这些数据表明海绵共生群落在贝加尔湖生态系统初级生产积累和碳循环中所起的作用。
