金黄精的生物合成由一个双模块非核糖体肽合成酶介导。
Chrysogine Biosynthesis Is Mediated by a Two-Module Nonribosomal Peptide Synthetase.
作者信息
Wollenberg Rasmus Dam, Saei Wagma, Westphal Klaus Ringsborg, Klitgaard Carina Sloth, Nielsen Kåre Lehmann, Lysøe Erik, Gardiner Donald Max, Wimmer Reinhard, Sondergaard Teis Esben, Sørensen Jens Laurids
机构信息
Department of Chemistry and Bioscience, Aalborg University , Fredrik Bajers Vej 7H, 9220 Aalborg Ø, Denmark.
Department of Biotechnology and Plant Health, NIBIO-Norwegian Institute of Bioeconomy Research , Høgskoleveien 7, 1430 Ås, Norway.
出版信息
J Nat Prod. 2017 Jul 28;80(7):2131-2135. doi: 10.1021/acs.jnatprod.6b00822. Epub 2017 Jul 14.
Production of chrysogine has been reported from several fungal genera including Penicillium, Aspergillus, and Fusarium. Anthranilic acid and pyruvic acid, which are expected precursors of chrysogine, enhance production of this compound. A possible route for the biosynthesis using these substrates is via a nonribosomal peptide synthetase (NRPS). Through comparative analysis of the NRPSs from genome-sequenced producers of chrysogine we identified a candidate NRPS cluster comprising five additional genes named chry2-6. Deletion of the two-module NRPS (NRPS14 = chry1) abolished chrysogine production in Fusarium graminearum, indicating that the gene cluster is responsible for chrysogine biosynthesis. Overexpression of NRPS14 enhanced chrysogine production, suggesting that the NRPS is the bottleneck in the biosynthetic pathway.
据报道，包括青霉属、曲霉属和镰刀菌属在内的几个真菌属均可产生金黄素。邻氨基苯甲酸和丙酮酸是预期的金黄素前体，可提高该化合物的产量。利用这些底物进行生物合成的一条可能途径是通过非核糖体肽合成酶（NRPS）。通过对已进行基因组测序的金黄素产生菌的NRPS进行比较分析，我们鉴定出一个候选NRPS基因簇，该基因簇包含另外五个名为chry2 - 6的基因。删除双模块NRPS（NRPS14 = chry1）会消除禾谷镰刀菌中金黄素的产生，这表明该基因簇负责金黄素的生物合成。NRPS14的过表达提高了金黄素的产量，这表明NRPS是生物合成途径中的瓶颈。
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