Heterologous Expression of the Core Genes in the Complex Fusarubin Gene Cluster of .

Through stepwise recreation of the biosynthetic gene cluster containing from , it was possible to produce the core scaffold compound of bostrycoidin, a red aza-anthraquinone pigment in . This was achieved through sequential transformation associated recombination (TAR) cloning of , , , and into the pESC-vector system, utilizing the inducible bidirectional galactose promoter for heterologous expression in . The production of the core metabolite bostrycoidin was investigated through triplicate growth cultures for 1-4 days, where the maximum titer of bostrycoidin was achieved after 2 days of induction, yielding 2.2 mg/L.