开发一种双重Taqman实时荧光定量PCR检测方法，用于从细菌培养物中快速鉴定灿烂弧菌相关菌株和河口弧菌菌株。
Development of a duplex Taqman real-time PCR assay for rapid identification of Vibrio splendidus-related and V. aestuarianus strains from bacterial cultures.
作者信息
Saulnier Denis, De Decker Sophie, Tourbiez Delphine, Travers Marie Agnès
机构信息
Ifremer, UMR 241 EIO, UPF-ILM-IRD, B.P. 49, 98719 Taravao, Tahiti, French Polynesia; Laboratoire d'Excellence "CORAIL", 98729, Moorea, French Polynesia.
Laboratoire de Génétique et Pathologie des Mollusques Marins, SG2M-RBE Ifremer, av du Mus de Loup, 17390 La Tremblade, French Polynesia.
出版信息
J Microbiol Methods. 2017 Sep;140:67-69. doi: 10.1016/j.mimet.2017.07.002. Epub 2017 Jul 12.
To enable the rapid and accurate identification of Vibrio splendidus-related and V. aestuarianus strains associated with Pacific cupped oyster Crassostrea gigas mortality, we developed a duplex Taqman real-time PCR assay and evaluated its efficacy. This technique proved to be rapid, sensitive, and specific and will be particularly valuable for epidemiologic studies.
为了能够快速准确地鉴定与太平洋牡蛎（Crassostrea gigas）死亡相关的灿烂弧菌（Vibrio splendidus）和河口弧菌（V. aestuarianus）菌株，我们开发了一种双重Taqman实时荧光定量PCR检测方法并评估了其有效性。该技术被证明具有快速、灵敏和特异的特点，对流行病学研究将具有特别重要的价值。
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