Department of Anatomy, Aichi Medical University, 1-1 Yazakokarimata, Nagakute, Aichi, 480-1195, Japan.
Department of Anatomy, Tokyo Medical University, 6-1-1, Shinjuku, Shinjuku-ku, Tokyo, 160-8402, Japan.
Sci Rep. 2017 Jul 14;7(1):5422. doi: 10.1038/s41598-017-05880-2.
Many studies on various organs have concluded that venous congestion (VC) causes severe organ dysfunction with elevation of oxidative stress relative to that of arterial ischaemia (AI). However, a comparison of the pathological effects of AI and VC on the testes has not been conducted. In this study, models of AI and VC and their reperfusion in rat testes, respectively, were developed and analysed. Testicular arteries or veins were interrupted for 6 h, re-perfused and kept for 4 weeks; the effects on the testes were then evaluated. Severe spermatogenic disturbances were observed at 4 weeks after reperfusion in AI but not in VC. At 6 h after blood flow interruption, oxidative stress was significantly increased and germ cells were severely damaged in AI compared with those in VC. RT-PCR analyses revealed that haem oxygenase-1, which exhibits anti-oxidative effects, and vascular endothelial growth factor-A, which exhibits vasculogenic effects, were significantly increased in VC but not in AI. Surprisingly, the results of our experiment in rat testes differed from those of experiments in previous studies performed in other organs. Oxidative stress in testes was more easily elevated by AI than it was by VC, explainable by the different experimental conditions.
许多关于各种器官的研究得出结论，静脉淤血（VC）导致严重的器官功能障碍，其氧化应激水平高于动脉缺血（AI）。然而，尚未对 AI 和 VC 对睾丸的病理影响进行比较。在这项研究中，分别建立和分析了大鼠睾丸的 AI 和 VC 模型及其再灌注。睾丸动脉或静脉阻断 6 小时，再灌注并保持 4 周；然后评估对睾丸的影响。再灌注 4 周后，AI 组出现严重的生精障碍，但 VC 组未见。在血流阻断后 6 小时，AI 组的氧化应激显著增加，与 VC 组相比，生殖细胞受到严重损伤。RT-PCR 分析显示，具有抗氧化作用的血红素加氧酶-1 和具有血管生成作用的血管内皮生长因子-A 在 VC 中显著增加，但在 AI 中没有增加。令人惊讶的是，我们在大鼠睾丸中的实验结果与之前在其他器官进行的实验结果不同。AI 比 VC 更容易引起睾丸氧化应激，这可以用不同的实验条件来解释。
