Menichini Francesco, Tundis Rosa, Loizzo Monica R, Bonesi Marco, D'Angelo Danila, Lombardi Pietro, Mastellone Vincenzo
a Department of Pharmacy , Health and Nutritional Sciences, University of Calabria , Rende , Italy and.
b Department of Veterinary Medicine and Animal Production , University of Naples Federico II , Naples , Italy.
J Enzyme Inhib Med Chem. 2016 Dec;31(6):1270-6. doi: 10.3109/14756366.2015.1115400. Epub 2015 Dec 8.
This study aimed to investigate the antidiabetic, antilipidaemic and antioxidant activities of Citrus medica cv Diamante (Rutaceae) hydroalcoholic (CD) peel extract in Zucker diabetic fatty (ZDF) rats. The ability of CD to protect against oxidative stress was investigated by using different in vitro assays and in vivo by using the reactive oxygen metabolites-derived compounds (d-ROMs) test and the biological antioxidant potential test (BAP). Two different doses of CD extract (300 and 600 mg/kg/die) were administered at ZDF rats for 4 weeks. CD reduced cholesterol and triglycerides levels. A dose-dependent effect on body weight and serum glucose levels was observed. A decrease of d-ROMs and an increase of BAP were recorded by using the dose of 600 mg/kg. The extract inhibited lipid peroxidation (IC50 value of 0.23 mg/ml). These findings suggest as an efficient phytotherapeutic approach in combating hyperlipidaemic and hyperglycaemic disorders.
本研究旨在探讨枸橼（芸香科）“钻石”品种水醇提取物（CD）对Zucker糖尿病肥胖（ZDF）大鼠的抗糖尿病、抗血脂和抗氧化活性。通过不同的体外试验研究CD对抗氧化应激的能力，并在体内通过活性氧代谢产物衍生化合物（d-ROMs）试验和生物抗氧化潜力（BAP）试验进行研究。给ZDF大鼠施用两种不同剂量的CD提取物（300和600mg/kg/天），持续4周。CD降低了胆固醇和甘油三酯水平。观察到对体重和血糖水平有剂量依赖性影响。使用600mg/kg剂量时，d-ROMs降低，BAP升高。该提取物抑制脂质过氧化（IC50值为0.23mg/ml）。这些发现表明，它是对抗高脂血症和高血糖症的一种有效植物治疗方法。
