Biomaterials, Biomechanics and Tissue Engineering Group, Department of Materials Science and Metallurgical Engineering, Technical University of Catalonia (UPC), EEBE, 08019, Av. Eduard Maristany 10-14, 08019, Barcelona, Spain.
Centre for Research in NanoEngineering (CRNE), UPC, EEBE, Av. Eduard Maristany 10-14, 08019, Barcelona, Spain.
Adv Healthc Mater. 2017 Oct;6(19). doi: 10.1002/adhm.201700327. Epub 2017 Jul 17.
The main drawbacks of cardiovascular bare-metal stents (BMS) are in-stent restenosis and stent thrombosis as a result of an incomplete endothelialization after stent implantation. Nano- and microscale modification of implant surfaces is a strategy to recover the functionality of the artery by stimulating and guiding molecular and biological processes at the implant/tissue interface. In this study, cobalt-chromium (CoCr) alloy surfaces are modified via direct laser interference patterning (DLIP) in order to create linear patterning onto CoCr surfaces with different periodicities (≈3, 10, 20, and 32 µm) and depths (≈20 and 800 nm). Changes in surface topography, chemistry, and wettability are thoroughly characterized before and after modification. Human umbilical vein endothelial cells' adhesion and spreading are similar for all patterned and plain CoCr surfaces. Moreover, high-depth series induce cell elongation, alignment, and migration along the patterned lines. Platelet adhesion and aggregation decrease in all patterned surfaces compared to CoCr control, which is associated with changes in wettability and oxide layer characteristics. Cellular studies provide evidence of the potential of DLIP topographies to foster endothelialization without enhancement of platelet adhesion, which will be of high importance when designing new BMS in the future.
心血管裸金属支架（BMS）的主要缺点是支架植入后内皮化不完全，导致支架内再狭窄和支架内血栓形成。通过对植入物表面进行纳米和微观尺度的修饰，可以通过刺激和引导植入物/组织界面处的分子和生物过程来恢复动脉的功能。在这项研究中，通过直接激光干涉图案化（DLIP）对钴铬（CoCr）合金表面进行修饰，以便在 CoCr 表面上创建具有不同周期性（≈3、10、20 和 32 µm）和深度（≈20 和 800 nm）的线性图案。在修饰前后对表面形貌、化学性质和润湿性进行了彻底的表征。与所有图案化和普通 CoCr 表面相比，人脐静脉内皮细胞的黏附和铺展相似。此外，高深度系列诱导细胞沿着图案线伸长、对齐和迁移。与 CoCr 对照相比，所有图案化表面的血小板黏附和聚集减少，这与润湿性和氧化层特性的变化有关。细胞研究为 DLIP 形貌促进内皮化而不增强血小板黏附的潜力提供了证据，这在未来设计新型 BMS 时非常重要。
