Rocquelin G, Yoyo N, Ducruet J M
Reprod Nutr Dev (1980). 1986;26(1A):97-112.
For 9 weeks, 80 male SPF Wistar rats were fed purified diets containing mixtures of vegetable oils (15% by weight) with different linoleic and linolenic contents. Diet (L+) contained large amounts of linoleic acid (53% of the total fatty acids), diet (L+Ln) contained the same amount of linoleic acid but also 10% of alpha-linolenic acid, diet (L-) supplied a low level of linoleic acid (12% of the total fatty acids) and so did diet (L-Ln) which also contained 10% of alpha-linolenic acid. The levels and fatty acid composition of heart phospholipids were determined. Liposomes, prepared from the total phospholipids extracted from rat hearts, were tested at different temperatures (15 to 50 degrees C) for their permeability to urea and fluidity; fluidity was monitored by fluorescence depolarization of 1,6-diphenyl-1,3,5-hexatriene (DPH). As already demonstrated, dietary alpha-linolenic acid substituted C22 polyunsaturated fatty acids of the linoleic family (n-6) for those of the linolenic acid family (n-3). This substitution remained high, even though linoleic acid represented more than 50% of the total dietary fatty acids. Diphosphatidylglycerol markedly increased in heart phospholipids of rats fed diets (L+) and (L+Ln). The changes in liposome permeability observed were rather well correlated with the unsaturation index of the phospholipid fatty acids, whereas fluidity changes were not. Fluidity decreased in liposomes of rats fed high levels of linoleic acid. Factors such as diphosphatidylglycerol or (n-3) fatty acid concentration in heart phospholipids could explain these results.
在9周的时间里，给80只雄性SPF级Wistar大鼠喂食含有不同亚油酸和亚麻酸含量的植物油混合物（占体重的15%）的纯化日粮。日粮（L+）含有大量亚油酸（占总脂肪酸的53%），日粮（L+Ln）含有相同量的亚油酸，但也含有10%的α-亚麻酸，日粮（L-）提供低水平的亚油酸（占总脂肪酸的12%），日粮（L-Ln）也是如此，其也含有10%的α-亚麻酸。测定了心脏磷脂的水平和脂肪酸组成。用从大鼠心脏中提取的总磷脂制备脂质体，在不同温度（15至50摄氏度）下测试其对尿素的渗透性和流动性；通过1,6-二苯基-1,3,5-己三烯（DPH）的荧光去极化监测流动性。如已证明的，日粮中的α-亚麻酸用亚麻酸家族（n-3）的多不饱和脂肪酸取代了亚油酸家族（n-6）的C22多不饱和脂肪酸。即使亚油酸占日粮总脂肪酸的50%以上，这种取代率仍然很高。在喂食日粮（L+）和（L+Ln）的大鼠心脏磷脂中，二磷脂酰甘油显著增加。观察到的脂质体渗透性变化与磷脂脂肪酸的不饱和度指数相当相关，而流动性变化则不然。喂食高水平亚油酸的大鼠的脂质体流动性降低。心脏磷脂中的二磷脂酰甘油或（n-3）脂肪酸浓度等因素可以解释这些结果。
