Rocquelin G, Guenot L, Astorg P O, David M
Station de Recherches sur la Qualite des Aliments de l'Homme, INRA, Dijon, France.
Lipids. 1989 Sep;24(9):775-80. doi: 10.1007/BF02544583.
Phospholipid content and fatty acid composition of human heart were determined on 36 biopsy specimens collected during open heart surgery. The main phospholipid classes, phosphatidylcholine (PC), phosphatidylethanolamine (PE), diphosphatidylglycerol (DPG), and sphingomyelin (SPH) were separated by HPLC, quantified, and converted to fatty acid methyl esters which were chromatographed on capillary GLC columns. Sex and age (mainly 40-70) of patients had no significant influence on the relative distribution of phospholipid classes and only a slight effect on fatty acid composition. Incorporation of trans 18:1 in phospholipid classes was low. cis and trans octadecenoic isomers seemed to be selectively incorporated, the delta 9 and delta 11 cis or trans isomers being predominant. Human and rat data were compared, and some species differences were noticed. In human PC, palmitic acid is higher and stearic acid much lower than in rat PC. Saturated dimethyl acetals (16:0 and 18:0) in PC and PE were greater for humans. Incorporation of 20:4 n-6 in human PE is higher than in rat PE.
对36份在心脏直视手术期间采集的活检标本测定了人心脏的磷脂含量和脂肪酸组成。主要的磷脂类别，即磷脂酰胆碱（PC）、磷脂酰乙醇胺（PE）、二磷脂酰甘油（DPG）和鞘磷脂（SPH），通过高效液相色谱法（HPLC）进行分离、定量，并转化为脂肪酸甲酯，然后在毛细管气相色谱柱上进行色谱分析。患者的性别和年龄（主要为40至70岁）对磷脂类别的相对分布没有显著影响，对脂肪酸组成只有轻微影响。反式18:1在磷脂类别中的掺入量较低。顺式和反式十八碳烯酸异构体似乎被选择性掺入，其中δ9和δ11顺式或反式异构体占主导。对人和大鼠的数据进行了比较，并注意到了一些物种差异。在人PC中，棕榈酸含量高于大鼠PC，而硬脂酸含量则远低于大鼠PC。人PC和PE中的饱和二甲基缩醛（16:0和18:0）含量更高。人PE中20:4 n-6的掺入量高于大鼠PE。
