Separation and quantification of heart and liver phospholipid classes by high-performance liquid chromatography using a new light-scattering detector.

This work describes a one-step separation of rat tissue phospholipid classes by high-performance liquid chromatography (HPLC) using a silica column and a new light-scattering detector (LSD). Complete separation of phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylserine, sphingomyelin, lysophosphatidylethanolamine, and lysophosphatidylcholine was obtained. Direct quantification was achieved after detector calibration for each phospholipid class. The detector response was shown to be linear within the ranges used. The LSD results agreed well with those obtained by phospholipid phosphorus assay. The present method was applied to rat heart and rat liver phospholipid analysis.