Juanéda P, Rocquelin G, Astorg P O
Institut National de la Recherche Agronomique, Station de Recherches sur la Qualité des Aliments de l'Homme, Dijon, France.
Lipids. 1990 Nov;25(11):756-9. doi: 10.1007/BF02544047.
This work describes a one-step separation of rat tissue phospholipid classes by high-performance liquid chromatography (HPLC) using a silica column and a new light-scattering detector (LSD). Complete separation of phosphatidylcholine, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, phosphatidylserine, sphingomyelin, lysophosphatidylethanolamine, and lysophosphatidylcholine was obtained. Direct quantification was achieved after detector calibration for each phospholipid class. The detector response was shown to be linear within the ranges used. The LSD results agreed well with those obtained by phospholipid phosphorus assay. The present method was applied to rat heart and rat liver phospholipid analysis.
本研究描述了一种使用硅胶柱和新型光散射检测器（LSD）通过高效液相色谱（HPLC）一步分离大鼠组织磷脂类别的方法。实现了磷脂酰胆碱、磷脂酰乙醇胺、二磷脂酰甘油、磷脂酰肌醇、磷脂酰丝氨酸、鞘磷脂、溶血磷脂酰乙醇胺和溶血磷脂酰胆碱的完全分离。对每种磷脂类别进行检测器校准后实现了直接定量。结果表明，检测器响应在所使用的范围内呈线性。LSD的结果与通过磷脂磷测定法获得的结果非常吻合。本方法应用于大鼠心脏和大鼠肝脏的磷脂分析。
