Department of Breast Cancer, Cancer Center, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.
Department of Nuclear Medicine, Guangdong General Hospital, Guangdong Academy of Medical Sciences, Guangzhou, China.
Int J Biol Macromol. 2017 Dec;105(Pt 1):346-353. doi: 10.1016/j.ijbiomac.2017.07.053. Epub 2017 Jul 16.
Long non-coding RNAs play a significant role in cancer metastasis. Studies have demonstrated that LncRNA NEAT1 promotes cancer progression. We aimed to explore whether NEAT1 regulated growth and invasion in breast cancer cells. We provided evidence that lncRNA NEAT1 was up-regulated in breast cancer cell lines and tissues. NEAT1 promoted invasion through inducing Epithelial-mesenchymal transition (EMT) and NEAT1 played a role in 5-fluorouracil (5-FU) resistance. In addition, we revealed a reciprocal repression between NEAT1 and miR-211. Furthermore, the EMT-inducer HMGA2 was identified as a down-stream target of miR-211. LncRNA NEAT1 induced EMT and 5-FU resistance through the miR-211/HMGA2 axis. Our findings suggest that lncRNA NEAT1 could be a new diagnostic biomarker and therapy target for breast cancer.
长非编码 RNA 在癌症转移中发挥重要作用。研究表明，LncRNA NEAT1 促进癌症进展。我们旨在探讨 NEAT1 是否调节乳腺癌细胞的生长和侵袭。我们提供的证据表明，lncRNA NEAT1 在乳腺癌细胞系和组织中上调。NEAT1 通过诱导上皮-间充质转化（EMT）促进侵袭，并且 NEAT1 在 5-氟尿嘧啶（5-FU）耐药中发挥作用。此外，我们揭示了 NEAT1 和 miR-211 之间的相互抑制关系。此外，EMT 诱导剂 HMGA2 被鉴定为 miR-211 的下游靶标。LncRNA NEAT1 通过 miR-211/HMGA2 轴诱导 EMT 和 5-FU 耐药。我们的研究结果表明，lncRNA NEAT1 可能成为乳腺癌的新诊断生物标志物和治疗靶点。
