Masaki Hideki, Nakauchi Hiromitsu
Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan.
Center for Stem Cell Biology and Regenerative Medicine, Institute of Medical Science, University of Tokyo, Tokyo 108-8639, Japan
Development. 2017 Jul 15;144(14):2544-2547. doi: 10.1242/dev.151183.
Interspecies chimeric assays are a valuable tool for investigating the potential of human stem and progenitor cells, as well as their differentiated progeny. This Spotlight article discusses the different factors that affect interspecies chimera generation, such as evolutionary distance, developmental timing, and apoptosis of the transplanted cells, and suggests some possible strategies to address them. A refined approach to generating interspecies chimeras could contribute not only to a better understanding of cellular potential, but also to understanding the nature of xenogeneic barriers and mechanisms of heterochronicity, to modeling human development, and to the creation of human transplantable organs.
种间嵌合体实验是研究人类干细胞和祖细胞及其分化后代潜能的重要工具。这篇聚焦文章讨论了影响种间嵌合体生成的不同因素，如进化距离、发育时间以及移植细胞的凋亡，并提出了一些可能的应对策略。一种改进的种间嵌合体生成方法不仅有助于更好地理解细胞潜能，还有助于理解异种屏障的本质和异时性机制、模拟人类发育以及创造可用于人类移植的器官。
