Joshi Robin, Rana Ajay, Kumar Vinay, Kumar Dharmesh, Padwad Yogendra S, Yadav Sudesh Kumar, Gulati Ashu
Food and Nutraceutical Division, Academy of Scientific and Innovative Research, CSIR-Institute of Himalayan Bioresource Technology, Palampur, Himachal Pradesh 176061 India.
Central University of Punjab, Bathinda, Punjab India.
J Food Sci Technol. 2017 Jun;54(7):1953-1963. doi: 10.1007/s13197-017-2631-7. Epub 2017 Apr 18.
Purple coloured tea shoot clones have gained interest due to high content of anthocyanins in addition to catechins. Transcript expression of genes encoding anthocyanidin reductase (), dihydroflavonol-4-reductase (), anthocyanidin synthase (), flavonol synthase () and leucoantho cyanidin reductase () enzymes in three new purple shoot tea clones compared with normal tea clone showed higher expression of , , and lower expression of and in purple shoot clones compared to normal clone. Expression pattern supported high content of anthocyanins in purple tea. Four anthocyanins (AN) were isolated and characterized by UPLC-ESI-QToF-MS/MS from IHBT 269 clone which recorded highest total anthocyanins content. Cyanidin-3---d-(6-()-coumaroyl) glucopyranoside (AN) showed highest in vitro antioxidant activity (IC = 25.27 ± 0.02 μg/mL and IC = 10.71 ± 0.01 μg/mL). Anticancer and immunostimulatory activities of cyanidin-3-glucoside (AN), cyanidin-3---d-(6-()-coumaroyl) glucopyranoside (AN), delphinidin-3---d-(6-()-coumaroyl) glucopyranoside (AN), cyanidin-3--(2---xylopyranosyl-6--acetyl)--glucopyranoside (AN) and crude anthocyanin extract (AN) showed high therapeutic perspective. Anthocyanins AN and crude extract AN showed cytotoxicity on C-6 cancer cells and high relative fluorescence units (RFU) at 200 μg/mL suggesting promising apoptosis induction activity as well as influential immunostimulatory potential. Observations demonstrate potential of purple anthocyanins enriched tea clone for exploitation as a nutraceutical product.
除儿茶素外，紫色茶树克隆体因花青素含量高而受到关注。与正常茶树克隆体相比，在三个新的紫色芽茶树克隆体中，编码花青素还原酶、二氢黄酮醇-4-还原酶、花青素合酶、黄酮醇合酶和无色花青素还原酶的基因转录表达显示，紫色芽克隆体中、和的表达较高，而和的表达较低。表达模式支持紫色茶中花青素含量高。从总花青素含量最高的IHBT 269克隆体中通过超高效液相色谱-电喷雾电离-四极杆飞行时间串联质谱法分离并鉴定了四种花青素（AN）。矢车菊素-3--d-（6-（）-香豆酰基）吡喃葡萄糖苷（AN）表现出最高的体外抗氧化活性（IC = 25.27±0.02μg/mL和IC = 10.71±0.01μg/mL）。矢车菊素-3-葡萄糖苷（AN）、矢车菊素-3--d-（6-（）-香豆酰基）吡喃葡萄糖苷（AN）、飞燕草素-3--d-（6-（）-香豆酰基）吡喃葡萄糖苷（AN）、矢车菊素-3--（2--木糖吡喃糖基-6--乙酰基）--吡喃葡萄糖苷（AN）和粗花青素提取物（AN）的抗癌和免疫刺激活性显示出很高的治疗前景。花青素AN和粗提取物AN对C-6癌细胞具有细胞毒性，在200μg/mL时具有高相对荧光单位（RFU），表明具有良好的凋亡诱导活性以及有影响力的免疫刺激潜力。观察结果表明，富含紫色花青素的茶树克隆体有作为营养保健品开发的潜力。
