Institute of Medical Microbiology and Hygiene, University Hospital Ulm, Albert-Einstein-Allee 23, 89081, Ulm, Germany.
Infection. 2013 Feb;41(1):159-66. doi: 10.1007/s15010-012-0301-2. Epub 2012 Aug 2.
In Germany, reliable data about the prevalence of urogenital Chlamydia trachomatis infections, causative genotypes, as well as corresponding clinical, demographic and behavioural information are sparse. We, therefore, performed a prospective prevalence study including 1,003 sexually active volunteers of a Southern German city.
Study participants completed a standardised questionnaire and provided first void urine samples for analysis. Our screening strategy included the performance of two nucleic acid amplification tests with different target genes, enabling the detection of the new Swedish variant of C. trachomatis (nvCT). Direct genotyping of positive specimens was performed by sequence analysis of the ompA gene.
The overall prevalence of C. trachomatis infection was 4.2 % in women and 4.6 % in men. A relatively high prevalence of 8.3 % was found in men older than 25 years. Never using condoms was an independent risk factor for infection. The most common symptom was discharge; however, 64.5 % of infected females and all of the infected men were asymptomatic, supporting the need for screening programmes. The most frequently encountered genotypes were E (46.5 %), F (20.9 %) and K (14.0 %). Since the nvCT was detected in one female student, this is one of the rare studies that reports on the molecular identification of nvCT apart from Sweden.
在德国，有关泌尿生殖沙眼衣原体感染的流行情况、致病基因型，以及相应的临床、人口统计学和行为信息的可靠数据较为匮乏。因此，我们开展了一项前瞻性流行性病学研究，纳入了德国南部一城市的 1003 名活跃性伴侣。
研究参与者完成了一份标准化问卷，并提供了首次排空的尿液样本进行分析。我们的筛查策略包括采用两种针对不同靶基因的核酸扩增检测，从而能够检测到新型瑞典变异沙眼衣原体（nvCT）。通过对 ompA 基因的序列分析对阳性样本进行直接基因分型。
女性和男性的沙眼衣原体感染总患病率分别为 4.2%和 4.6%。25 岁以上男性的患病率相对较高，为 8.3%。从不使用安全套是感染的独立危险因素。最常见的症状是分泌物；然而，64.5%的受感染女性和所有受感染男性无症状，这支持了筛查计划的必要性。最常见的基因型是 E（46.5%）、F（20.9%）和 K（14.0%）。由于在一名女学生中检测到了 nvCT，这是除瑞典以外少数报告分子鉴定 nvCT 的研究之一。
