Hubálek Zdenek, Juricová Zina, Straková Petra, Blazejová Hana, Betásová Lenka, Rudolf Ivo
1 Institute of Vertebrate Biology , Academy of Sciences, v.v.i., Brno, Czech Republic .
2 Department of Experimental Biology, Faculty of Science, Masaryk University , Brno, Czech Republic .
Vector Borne Zoonotic Dis. 2017 Sep;17(9):654-657. doi: 10.1089/vbz.2017.2109. Epub 2017 Jul 20.
Antibodies to West Nile virus (WNV) were examined by plaque-reduction neutralization test in the blood sera of 1023 wild artiodactyls: 105 roe deer (Capreolus capreolus), 148 red deer (Cervus elaphus), 287 fallow deer (Dama dama), 71 mouflons (Ovis musimon), and 412 wild boars (Sus scrofa), sampled in South Moravian district of Breclav (Czech Republic) in the years 1990-2008. Neutralizing antibodies were detected in 5.9% of wild ruminants (4.8% roe deer, 4.1% red deer, 6.3% fallow deer, 9.9% mouflons) and 4.1% of wild boars, with titers ranging between 1:20 and 1:320. The results indicate that WNV has circulated in wild artiodactyls at a variable frequency during the years in the area.
采用蚀斑减少中和试验对1023头野生偶蹄动物血清中的西尼罗河病毒(WNV)抗体进行检测,这些动物包括105头狍(Capreolus capreolus)、148头马鹿(Cervus elaphus)、287头黇鹿(Dama dama)、71头摩弗伦羊(Ovis musimon)和412头野猪(Sus scrofa),于1990年至2008年在捷克共和国布雷斯拉夫的南摩拉维亚地区采集。在5.9%的野生反刍动物(4.8%的狍、4.1%的马鹿、6.3%的黇鹿、9.9%的摩弗伦羊)和4.1%的野猪中检测到中和抗体,效价在1:20至1:320之间。结果表明,在该地区这些年份里,WNV在野生偶蹄动物中以不同频率传播。