上颌窦底提升后间充质干细胞条件培养液促进骨再生的临床研究。
Clinical Study of Bone Regeneration by Conditioned Medium From Mesenchymal Stem Cells After Maxillary Sinus Floor Elevation.
机构信息
*Associate Professor, Division of Reconstructive Surgery and Oral and Maxillofacial Region, Niigata University Graduate School of Medical and Dental Sciences, Niigata, Japan; Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan. †Graduate Student, Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan. ‡Assistant Professor, Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan. §Professor, Department of Oral and Maxillofacial Surgery, Nagoya University Graduate School of Medicine, Nagoya, Japan.
出版信息
Implant Dent. 2017 Aug;26(4):607-612. doi: 10.1097/ID.0000000000000618.
OBJECTIVE
This clinical study was undertaken to evaluate the safety of use of the secretome of bone marrow-derived mesenchymal stem cells (MSC-CM) for maxillary sinus floor elevation (SFE).
MATERIALS AND METHODS
MSC-CM was prepared from conditioned medium from human bone marrow-derived MSCs. Six partially edentulous patients were enrolled in the study. MSC-CM was mixed with porous beta-tricalcium phosphate (β-TCP) and implanted in 4 patients (experimental group), whereas only β-TCP was implanted in the other 2 patients (control group). Six months after SFE, bone biopsies and histological assessments were performed.
RESULTS
Bone formation was clinically confirmed in all cases. Although Hounsfield units in computed tomography images were not significantly different between the groups, histological analysis revealed a significant difference in newly formed bone area between the groups. In particular, bone volume in the center of the augmented area was significantly greater in the MSC-CM group. Newly formed bone consisted of lamellar bone in the MSC-CM group but woven bone in the β-TCP group.
CONCLUSION
The secretome of bone marrow-derived mesenchymal stem cells (MSC-CM) was used safely and has great osteogenic potential for regenerative medicine of bone.
目的
本临床研究旨在评估骨髓间充质干细胞（MSC-CM）分泌液用于上颌窦底提升（SFE）的安全性。
材料和方法
MSC-CM 是从人骨髓间充质干细胞的条件培养基中制备的。本研究纳入了 6 名部分无牙患者。将 MSC-CM 与多孔 β-磷酸三钙（β-TCP）混合并植入 4 名患者（实验组），而另外 2 名患者仅植入 β-TCP（对照组）。SFE 后 6 个月，进行骨活检和组织学评估。
结果
所有病例均临床证实有骨形成。尽管两组的 CT 图像中的 Hounsfield 单位没有显著差异，但组织学分析显示两组新形成骨面积有显著差异。特别是，增强区中心的骨体积在 MSC-CM 组中显著更大。新形成的骨在 MSC-CM 组中为板层骨，而在 β-TCP 组中为编织骨。
结论
骨髓间充质干细胞（MSC-CM）的分泌液安全且具有很强的成骨潜力，可用于骨再生医学。
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