Abdul-Karim F W, Dahms B B, Velasco M E, Rodman H M
Arch Pathol Lab Med. 1986 Jul;110(7):602-6.
The incidence of fasting hyperglycemia and diabetes mellitus (DM) in older patients with cystic fibrosis (CF) is reported to be 8%, but few quantitative studies of the islets of Langerhans in this disease have been done. Three groups of patients were studied in this morphometric autopsy analysis: patients with CF and insulin-dependent DM (n = 7), normoglycemic patients with CF (n = 4), and age-matched adolescents and young-adult controls (n = 11). The islets of Langerhans were stained with immunoperoxidase for insulin, glucagon, and somatostatin. The percentage of the total islet surface area occupied by each immunoperoxidase positive cell type was determined by a point-counting method. The mean percent surface area occupied by insulin-producing cells (28.3%) in diabetics with CF was significantly less than normoglycemics with CF (46.7%) and controls (53.4%). The mean percent surface area occupied by glucagon-producing cells was similar in all three groups: 21.9% in CF diabetics, 25.4% in normoglycemics with CF, and 22.4% in controls. The mean percent surface area occupied by somatostatin was increased in both CF groups compared with controls: diabetics with CF, 29.3%; normoglycemics with CF, 26.2%; and controls, 15.5%. These findings correlate with published clinical endocrine studies of hyperglycemia in CF. Endocrine cell quantitation in diabetics with CF differs from that in both juvenile (type 1) and adult-onset (type 2) DM.
据报道，老年囊性纤维化（CF）患者中空腹血糖过高和糖尿病（DM）的发病率为8%，但针对该疾病中胰岛的定量研究较少。在这项形态计量学尸检分析中，研究了三组患者：患有CF且依赖胰岛素的糖尿病患者（n = 7）、血糖正常的CF患者（n = 4）以及年龄匹配的青少年和青年成人对照组（n = 11）。用免疫过氧化物酶对胰岛进行胰岛素、胰高血糖素和生长抑素染色。通过点计数法确定每种免疫过氧化物酶阳性细胞类型所占胰岛总面积的百分比。CF糖尿病患者中产生胰岛素的细胞所占平均表面积百分比（28.3%）显著低于CF血糖正常者（46.7%）和对照组（53.4%）。三组中产生胰高血糖素的细胞所占平均表面积百分比相似：CF糖尿病患者中为21.9%，CF血糖正常者中为25.4%，对照组中为22.4%。与对照组相比，两组CF患者中生长抑素所占平均表面积百分比均增加：CF糖尿病患者为29.3%，CF血糖正常者为26.2%，对照组为15.5%。这些发现与已发表的关于CF患者高血糖的临床内分泌研究结果相关。CF糖尿病患者的内分泌细胞定量与青少年（1型）和成人发病（2型）糖尿病患者均不同。
