Departamento de Microbiología del Suelo y Sistemas Simbióticos, Estación Experimental del Zaidín, Consejo Superior de Investigaciones Científicas, calle Profesor Albareda 1, 18008, Granada, Spain.
Departamento de Microbiología y Genética, Lab. 209. Universidad de Salamanca, edificio Departamental de Biología, Campus M. Unamuno, Salamanca, Spain.
Sci Rep. 2017 Jul 20;7(1):6008. doi: 10.1038/s41598-017-06112-3.
After a forest wildfire, the microbial communities have a transient alteration in their composition. The role of the soil microbial community in the recovery of an ecosystem following such an event remains poorly understood. Thus, it is necessary to understand the plant-microbe interactions that occur in burned soils. By high-throughput sequencing, we identified the main bacterial taxa of burnt holm-oak rhizosphere, then we obtained an isolate collection of the most abundant genus and its growth promoting activities were characterised. 16S rRNA amplicon sequencing showed that the genus Arthrobacter comprised more than 21% of the total community. 55 Arthrobacter strains were isolated and characterized using RAPDs and sequencing of the almost complete 16S rRNA gene. Our results indicate that isolated Arthrobacter strains present a very high genetic diversity, and they could play an important ecological role in interaction with the host plant by enhancing aerial growth. Most of the selected strains exhibited a great ability to degrade organic polymers in vitro as well as possibly presenting a direct mechanism for plant growth promotion. All the above data suggests that Arthrobacter can be considered as an excellent PGP rhizobacterium that may play an important role in the recovery of burned holm-oak forests.
森林野火后，微生物群落的组成会发生短暂变化。在这种情况下，土壤微生物群落对生态系统恢复的作用仍知之甚少。因此，有必要了解在燃烧土壤中发生的植物-微生物相互作用。通过高通量测序，我们确定了燃烧的油橄榄根际的主要细菌分类群，然后我们获得了最丰富属的分离物集，并对其生长促进活性进行了表征。16S rRNA 扩增子测序表明，节杆菌属占总群落的 21%以上。使用 RAPD 和几乎完整的 16S rRNA 基因测序对 55 株节杆菌进行了分离和表征。我们的结果表明，分离的节杆菌菌株具有非常高的遗传多样性，它们可能通过促进空中生长与宿主植物进行重要的生态相互作用。所选菌株中的大多数在体外具有很强的降解有机聚合物的能力，并且可能具有直接的植物生长促进机制。所有上述数据表明，节杆菌可以被认为是一种优秀的 PGPR 根际细菌，可能在燃烧的油橄榄森林恢复中发挥重要作用。
