Institute of Pathology, Ulm University, Ulm, Germany.
Institute of Medical Systems Biology, Ulm University, Ulm, Germany.
Sci Rep. 2017 Jul 20;7(1):5963. doi: 10.1038/s41598-017-05116-3.
Gastrointestinal (g.i.) large cell lymphoma is currently regarded as diffuse large B-cell lymphoma (DLBCL) despite a more favorable clinical outcome compared to other DLBCL. Cluster analyses on a transcriptome signature of NF-κB target genes of 30 g.i. marginal zone B-cell lymphomas (MZBL; 8 g.i. MZBL, 22 large cell MZBL - among them 9 with coexisting small cell component) and 6 DLBCL (3 activated B-cell like (ABC), 3 germinal center-like (GCB)) reveals a distinct pattern. The distinctiveness of large cell MZBL samples is further confirmed by a cohort of 270 available B-cell lymphoma and B-cell in silico profiles. Of the NF-κB genes analyzed, c-REL was overexpressed in g.i. MZBL. c-REL amplification was limited to 6/22 large cell MZBL including the large cell component of 2/9 composite small cell/large cell lymphomas, and c-Rel protein expression was found in the large cell compartment of composite lymphomas. Classification experiments on DLBCL and large cell MZBL profiles support the concept that the large cell MZBL is a distinct type of B-cell lymphoma.
胃肠道（GI）大细胞淋巴瘤目前被认为是弥漫性大 B 细胞淋巴瘤（DLBCL），尽管与其他 DLBCL 相比，其临床结局更为有利。对 30 例 GI 边缘区 B 细胞淋巴瘤（MZBL；8 例 GI MZBL，22 例大细胞 MZBL - 其中 9 例伴有共存的小细胞成分）和 6 例 DLBCL（3 例激活 B 细胞样（ABC），3 例生发中心样（GCB））的 NF-κB 靶基因转录组特征进行聚类分析，揭示了一种独特的模式。270 例可用的 B 细胞淋巴瘤和 B 细胞的队列进一步证实了大细胞 MZBL 样本的独特性。在所分析的 NF-κB 基因中，c-REL 在 GI MZBL 中过表达。c-REL 扩增仅限于 6/22 例大细胞 MZBL，包括 2/9 例复合小细胞/大细胞淋巴瘤的大细胞成分，并且在复合淋巴瘤的大细胞区室中发现了 c-Rel 蛋白表达。对 DLBCL 和大细胞 MZBL 图谱的分类实验支持这样的概念，即大细胞 MZBL 是一种独特的 B 细胞淋巴瘤类型。
