Zhao Yan, Zhou Long-Fang, Yang Hong
Department of Ophthalmology, Tongji Hospital, Tongji Medical College of Huazhong University of Science and Technology, Wuhan 430030, Hubei Province, China.
Department of Fundus Diseases, Wuhan Aier Eye Hospital, Wuhan 430060, Hubei Province, China.
Int J Ophthalmol. 2017 Jun 18;10(6):840-846. doi: 10.18240/ijo.2017.06.03. eCollection 2017.
To investigate the changes in vitreous gel structure after lens extirpation combined with anterior vitrectomy in rabbit eyes.
Twenty-eight chinchilla rabbits were divided into three groups. The control group (Group I) included 16 eyes from eight rabbits who did not receive any treatment. Group II included 20 eyes from 10 rabbits that underwent lens aspiration only. Group III included 20 eyes from 10 rabbits that underwent lens aspiration combined with posterior capsulotomy and anterior vitrectomy. Eyes were harvested on the 30 and 60 day postoperatively, respectively. Changes in vitreous gel stretch length due to gravity and the rate of vitreous liquefaction were observed. The collagen content in the vitreous body was examined using the L-hydroxyproline test. Electronic microscopic images were obtained from each eyeball.
On both the 30 and 60 day postoperatively, the vitreous gel length of group III was significantly shorter than group I and group II (<0.05), while the rate of liquefaction of the vitreous body in group III was significantly higher than group I and group II (<0.05). The collagen content in group III was also higher than that in group I and group II (<0.05).
Loss of vitreous gel mass is more likely to occur in the eyes of rabbits receiving anterior vitrectomy. Lensectomy combined with anterior vitrectomy may damage the stable three-dimensional mesh structure of collagen, which could aggravate vitreous gel liquefaction.
研究兔眼晶状体摘除联合前部玻璃体切除术后玻璃体凝胶结构的变化。
28只龙猫分为三组。对照组（I组）包括8只未接受任何治疗的兔子的16只眼。II组包括10只仅行晶状体抽吸术的兔子的20只眼。III组包括10只行晶状体抽吸联合后囊切开及前部玻璃体切除术的兔子的20只眼。分别在术后30天和60天摘取眼球。观察玻璃体凝胶因重力作用产生的伸展长度变化及玻璃体液化率。采用L-羟脯氨酸检测法检测玻璃体中的胶原蛋白含量。从每个眼球获取电子显微镜图像。
术后30天和60天，III组的玻璃体凝胶长度均显著短于I组和II组（<0.05），而III组的玻璃体液化率显著高于I组和II组（<0.05）。III组的胶原蛋白含量也高于I组和II组（<0.05）。
接受前部玻璃体切除术的兔眼更易发生玻璃体凝胶质量损失。晶状体切除术联合前部玻璃体切除术可能会破坏胶原蛋白稳定的三维网状结构，从而加重玻璃体凝胶液化。
