Tang Qi, Chen Chang, Wang Xiaqi, Li Wei, Zhang Yan, Wang Muyao, Jing Wei, Wang Hang, Guo Weihua, Tian Weidong
State Key Laboratory of Oral Diseases, West China Hospital of Stomatology, Sichuan University, Chengdu 610041, P.R. China.
Int J Mol Med. 2017 Sep;40(3):713-720. doi: 10.3892/ijmm.2017.3073. Epub 2017 Jul 19.
Adipose tissue engraftment has become a well-established therapy in plastic and reconstructive surgery used to restore age-related or injury-related soft tissue loss. However, the unpredictable absorption rates limit its further application. Some clinicians have noted that more optimal aesthetic results are achieved when botulinum toxin A (BoNTA) is applied prior to adipose tissue grafting. In the present study, we transplanted allogeneic adipose tissue treated with or without BoNTA in SD rats in vivo. We subsequently evaluated the survival rate (weight, volume, apoptosis and cellular integrity) and revascularization of the adipose tissue. The results revealed that BoNTA improved the long-term weight and volume retention of the graft, and preserved cellular integrity. BoNTA significantly increased the expression levels of CD31 and vascular endothelial growth factor (VEGF), suggesting enhanced vasodilation and endothelial cell proliferation. In vitro, adipose-derived stem cells (ASCs) were isolated, identified and induced to proliferate and differentiate with or without BoNTA. Furthermore, to evaluate the proliferative, adipogenic and angiogenic ability of the ASCs, CCK-8 assay and Oil Red O staining were conducted. Gene and protein expression levels were analyzed by RT-qPCR and western blot analysis. The results revealed that 8x10-2 U/ml BoNTA as the optimal dose increased ASC proliferation and adipogenic differentiation capacity, as well as the expression level of the key cytokine of angiogenesis. On the whole, our findings indicate that BoNTA improves adipose tissue engraftment and promotes ASC regeneration, which could benefit future clinical applications.
脂肪组织移植已成为整形和重建外科中一种成熟的治疗方法，用于修复与年龄相关或与损伤相关的软组织损失。然而，不可预测的吸收率限制了其进一步应用。一些临床医生指出，在脂肪组织移植前应用A型肉毒杆菌毒素（BoNTA）可获得更理想的美学效果。在本研究中，我们在体内将经或未经BoNTA处理的同种异体脂肪组织移植到SD大鼠体内。随后，我们评估了脂肪组织的存活率（重量、体积、凋亡和细胞完整性）以及血管再生情况。结果显示，BoNTA提高了移植物的长期重量和体积保留率，并保持了细胞完整性。BoNTA显著提高了CD31和血管内皮生长因子（VEGF）的表达水平，表明血管舒张和内皮细胞增殖增强。在体外，分离、鉴定脂肪来源干细胞（ASC），并在有或无BoNTA的情况下诱导其增殖和分化。此外，为评估ASC的增殖、成脂和血管生成能力，进行了CCK-8检测和油红O染色。通过RT-qPCR和蛋白质印迹分析来分析基因和蛋白质表达水平。结果显示，8×10-2 U/ml BoNTA作为最佳剂量可提高ASC的增殖和成脂分化能力，以及血管生成关键细胞因子的表达水平。总体而言，我们的研究结果表明，BoNTA可改善脂肪组织移植并促进ASC再生，这可能有利于未来的临床应用。
