Mazloum H, Totten P A, Brooks G F, Dawson C R, Falkow S, James J F, Knapp J S, Koomey J M, Lammel C J, Peters D
J Infect Dis. 1986 Aug;154(2):212-24. doi: 10.1093/infdis/154.2.212.
Seven isolates of an unusual Neisseria sp. were obtained from eye cultures of children in two rural Egyptian villages. These Neisseria utilized only glucose, they exhibited a positive reaction when tested with antisera to crude antigen from Neisseria meningitidis and N. gonorrhoeae, and they did not react with the fluorescent antibody tests for N. gonorrhoeae or with the monoclonal antibodies used to serotype gonococci. The Egyptian isolates had colony morphology more typical of meningococci than gonococci and showed opaque and transparent colony variants. On SDS-PAGE, the major outer-membrane proteins had different patterns than those noted for comparable proteins of meningococci and gonococci; heat-modifiable outer-membrane proteins were present. Four of the six isolates examined had cryptic plasmids of 2.8 megadaltons, which were slightly larger than the cryptic plasmid of N. gonorrhoeae. These plasmids were homologous to the gonococcal cryptic plasmid, but had different restriction enzyme fragment patterns. The DNA from the Egyptian isolates, like DNA from N. meningitidis but unlike DNA from N. gonorrhoeae, could be cut with the restriction enzyme HaeIII. The frequency of transformation into a temperature-sensitive mutant of N. gonorrhoeae was 0.2 for the Egyptian isolates and 0.1 for N. meningitidis, a frequency that was 5-10-fold lower than that for the N. gonorrhoeae control isolates. Whole-cell DNA from the Egyptian isolates showed 68%-73% homology with N. gonorrhoeae and 57%-63% with N. meningitidis. On the basis of our observations, the Egyptian isolates are distinct from N. meningitidis and may represent a variant of N. gonorrhoeae. We suggest that the isolates be called Neisseria gonorrhoeae ssp. kochii.
从埃及两个乡村儿童的眼部分离物中获得了7株不寻常的奈瑟菌属菌株。这些奈瑟菌仅利用葡萄糖，用针对脑膜炎奈瑟菌和淋病奈瑟菌粗抗原的抗血清检测时呈阳性反应，且它们与淋病奈瑟菌的荧光抗体试验或用于淋病双球菌血清分型的单克隆抗体不发生反应。埃及分离株的菌落形态比淋病奈瑟菌更具脑膜炎奈瑟菌的典型特征，并呈现出不透明和透明的菌落变体。在十二烷基硫酸钠-聚丙烯酰胺凝胶电泳（SDS-PAGE）上，主要外膜蛋白的模式与脑膜炎奈瑟菌和淋病奈瑟菌的相应蛋白不同；存在热可修饰的外膜蛋白。所检测的6株分离株中有4株含有2.8兆道尔顿的隐蔽质粒，略大于淋病奈瑟菌的隐蔽质粒。这些质粒与淋病奈瑟菌的隐蔽质粒同源，但具有不同的限制性酶切片段模式。埃及分离株的DNA，与脑膜炎奈瑟菌的DNA一样但与淋病奈瑟菌的DNA不同，可用限制性酶HaeIII切割。埃及分离株转化为淋病奈瑟菌温度敏感突变体的频率为0.2，脑膜炎奈瑟菌为0.1，该频率比淋病奈瑟菌对照分离株低5至10倍。埃及分离株的全细胞DNA与淋病奈瑟菌的同源性为68%-73%，与脑膜炎奈瑟菌的同源性为57%-63%。根据我们的观察，埃及分离株与脑膜炎奈瑟菌不同，可能代表淋病奈瑟菌的一个变种。我们建议将这些分离株称为淋病奈瑟菌科赫亚种。
