School of Chemistry and Chemical Engineering, University of Jinan, Jinan 250022, China.
Shandong Provincial Key Laboratory of Preparation and Measurement of Building Materials, University of Jinan, Jinan 250022, China.
Biosens Bioelectron. 2018 Jan 15;99:8-13. doi: 10.1016/j.bios.2017.07.031. Epub 2017 Jul 13.
In this paper, a dual-target electrochemical aptasensor has been developed for simultaneous detection of carcinoembryonic antigen and mucin-1 based on metal ion electrochemical labels and Ru(NH) electronic wires. When targets are present, the interaction between targets and their respective aptamers leads to the dissociation of double-strand DNA because the targets have higher affinity to its aptamer than the complementary strand. And the qualitative and quantitative analyses of the two targets are realized by the differential pulse voltammetry (DPV) peaks generated by metal ion electrochemical labels. For the effective loading of a large number of metal ions, Au/bovine serum albumin (Au/BSA) nanospheres are employed as carriers to develop Au/BSA-metal ions. After Ru(NH) complexes are embedded into double-strand DNA to form the electronic wires, the electrical conductivity and the electron transfer of the detection system are greatly improved. The detection limit of the proposed assay was calculated as 3.33fM ranging from 0.01pM to 100nM. Therefore, this novel sensing assay provides a new and sensitive platform for detecting several targets simultaneously in biochemical research and clinical diagnosis.
本文基于金属离子电化学标记物和 Ru(NH)电子线，开发了一种用于同时检测癌胚抗原和黏蛋白 1 的双靶电化学适体传感器。当存在靶标时，由于靶标与各自适体的相互作用，导致双链 DNA 的解离，因为靶标与适体的亲和力高于互补链。通过金属离子电化学标记物产生的差分脉冲伏安法（DPV）峰实现了两种靶标的定性和定量分析。为了有效负载大量金属离子，采用金/牛血清白蛋白（Au/BSA）纳米球作为载体来制备 Au/BSA-金属离子。Ru(NH)复合物嵌入双链 DNA 形成电子线后，检测系统的导电性和电子转移得到了极大的改善。所提出的测定方法的检测限计算为 3.33fM，范围从 0.01pM 到 100nM。因此，这种新型传感分析为生物化学研究和临床诊断中同时检测多个靶标提供了一个新的、灵敏的平台。
