Giorgi Annamaria, Bassoli Angela, Borgonovo Gigliola, Panseri Sara, Manzo Alessandra, Pentimalli Daniela, Schiano Moriello Aniello, De Petrocellis Luciano
Centre of Applied Studies for the Sustainable Management and Protection of Mountain Areas (Ge.S.Di.Mont.), University of Milan, Via Morino 8, 25048 Edolo, Brescia, Italy.
Department of Food, Environmental and Nutritional Sciences, University of Milan, Via Celoria 2, 20133 Milan, Italy.
Phytomedicine. 2017 Aug 15;32:80-87. doi: 10.1016/j.phymed.2017.04.012. Epub 2017 May 1.
Waldheimia glabra (Decne.) Regel is a wild plant from the Himalayan Mountains, commonly known as Smooth Ground Daisy. This plant is traditionally used by local populations in religious rituals (incense) or in traditional herbal medicine to treat skin diseases, headache, joint pain and fever. In literature few data are available on the investigation of this aromatic plant.
The present work aims at deepening knowledge about the chemical composition of W. glabra extracts and incense, as well as its activity on TRP ion channels.
Extracts and incense of W. glabra were analyzed by using HS-SPME GC/MS, GC/MS and NMR analysis. Tests on the activity of W. glabra extracts and isolated compounds (+)-ludartin 1 and B-ring-homo-tonghaosu 2 on TRP channels were also performed.
Some extracts and pure compounds from W. glabra showed an interesting activity in terms of efficacy and potency on rat TRPA1, an ion channel involved in several sensory mechanisms, including pungency, environmental irritation and pain perception. Activity is discussed and compared with that of other known TRPA1 natural agonists with different chemical structures. All compounds showed only a negligible inhibition activity on rat TRPM8 ion channel.
Our findings demonstrate that W. glabra is involved in the receptor activation mechanism and therefore represents a new natural product potentially useful in pharmaceutical and agrifood research.
光叶岩黄芪（Waldheimia glabra (Decne.) Regel）是一种来自喜马拉雅山脉的野生植物，俗称光叶地菊。这种植物在当地传统上用于宗教仪式（焚香）或传统草药中，以治疗皮肤病、头痛、关节疼痛和发烧。关于这种芳香植物的研究，文献中可用的数据很少。
本研究旨在加深对光叶岩黄芪提取物和香的化学成分及其对瞬时受体电位（TRP）离子通道活性的了解。
采用顶空固相微萃取气相色谱/质谱联用（HS-SPME GC/MS）、气相色谱/质谱联用（GC/MS）和核磁共振（NMR）分析对光叶岩黄芪的提取物和香进行分析。还对光叶岩黄芪提取物和分离出的化合物（+）-鲁达汀1和B环高同蒿素2对TRP通道的活性进行了测试。
光叶岩黄芪的一些提取物和纯化合物对大鼠TRPA1（一种参与多种感觉机制，包括辛辣味、环境刺激和疼痛感知的离子通道）表现出有趣的效力和功效活性。对该活性进行了讨论，并与其他具有不同化学结构的已知TRPA1天然激动剂的活性进行了比较。所有化合物对大鼠TRPM8离子通道仅表现出可忽略不计的抑制活性。
我们的研究结果表明，光叶岩黄芪参与受体激活机制，因此代表了一种可能在制药和农业食品研究中有用的新天然产物。
