Univ. Lille, Inserm, CHU Lille, U1008 - Controlled Drug Delivery Systems and Biomaterials, Lille, France.
Univ. Lille, CNRS, INRA, ENSCL UMR8207, UMET - Unité Matériaux et Transformations, F-59000 Lille, France.
Carbohydr Polym. 2017 Oct 1;173:535-546. doi: 10.1016/j.carbpol.2017.06.026. Epub 2017 Jun 7.
Chitosan (CS) presents antibacterial, mucoadhesive and hemostatic properties and is an ideal candidate for wound dressing applications. This work reports the development of sponge-like materials obtained from physical hydrogels after the interaction between CS and a β-cyclodextrin polymer (PCD) in acidic conditions to provoke immediate gelation. Characterization consisted of zeta potential (ZP) measurements, rheology analysis, Fourier transform infrared (FTIR), Raman spectroscopy, wide angle X-ray scattering (WAXS) and scanning electron microscopy (SEM). Swelling behavior, cytotoxicity, drug sorption and drug delivery properties of sponges were assessed. ZP indicated that CS and PCD presented opposite charges needed for physical crosslinking. Rheology, swelling, and cytotoxicity of sponges depended on their CS:PCD weight ratios. Increasing PCD in the mixture delayed the gel time, reduced the swelling and increased the cytotoxicity. FTIR and Raman confirmed the physical crosslinking between CS and PCD through ionic interactions, and WAXS showed the amorphous state of the sponges. Finally, the efficiency of chlorhexidine loaded sponge against S. aureus bacteria was proved for up to 30days in agar diffusion tests.
壳聚糖（CS）具有抗菌、粘膜粘附和止血性能，是伤口敷料应用的理想候选材料。本工作报道了在酸性条件下 CS 与β-环糊精聚合物（PCD）相互作用后获得的海绵状材料的发展，这种相互作用引发了立即胶凝。表征包括zeta 电位（ZP）测量、流变学分析、傅里叶变换红外（FTIR）、拉曼光谱、广角 X 射线散射（WAXS）和扫描电子显微镜（SEM）。评估了海绵的溶胀行为、细胞毒性、药物吸附和药物传递性能。ZP 表明 CS 和 PCD 具有物理交联所需的相反电荷。海绵的流变学、溶胀和细胞毒性取决于它们的 CS:PCD 重量比。混合物中 PCD 的增加会延迟凝胶时间、减少溶胀并增加细胞毒性。FTIR 和拉曼证实了 CS 和 PCD 之间通过离子相互作用的物理交联，WAXS 显示了海绵的无定形状态。最后，在琼脂扩散试验中证明了负载氯己定的海绵对金黄色葡萄球菌的效率可持续长达 30 天。
