壳聚糖交联焦硫酸钠串联超声纳米包封羟基酪醇：技术特性、释放和抗增殖性能。

Nanoencapsulation of hydroxytyrosol in chitosan crosslinked with sodium bisulfate tandem ultrasonication: Techno-characterization, release and antiproliferative properties.

机构信息

Department of Food Science and Technology, University of Kashmir, Hazratbal, Srinagar 190006, Jammu and Kashmir, India.

出版信息

Ultrason Sonochem. 2022 Jan;82:105900. doi: 10.1016/j.ultsonch.2021.105900. Epub 2021 Dec 28.

This research includes production of chitosan nanocapsules through ionic gelation with sodium bisulfate for nanoencapsulation of hydroxytyrosol (HT) using ultrasonication in tandem. The resulting nanocapsules encapsulating HT were analyzed for particle size, ζ-potential, packaging characteristics, FESEM, ATR-FTIR, XRD, DSC, in vitro release, antioxidant potential and antiproliferative properties. The nanocapsules (size 119.50-365.21 nm) were spherical to irregular shaped with positive ζ-potential (17.50-18.09 mV). The encapsulation efficiency of 5 mg/g HT (HTS1) and 20 mg/g HT (HTS2) was 77.13% and 56.30%, respectively. The nanocapsules were amorphous in nature with 12.34% to 15.48% crystallinity and crystallite size between 20 nm and 27 nm. Formation of nanocapsules resulted in increasing the glass transition temperature. HTS2 delivered 67.12% HT (HTS1 58.89%) at the end of the simulated gastrointestinal digestion. The nanoencapsulated HT showed higher antioxidant and antiproliferative (against A549 and MDA-MB-231 cancer cell lines) properties than the free HT.

本研究采用超声串联离子凝胶法，用硫酸氢钠制备壳聚糖纳米胶囊，对羟基酪醇（HT）进行纳米包封。对包封 HT 的纳米胶囊进行粒径、ζ-电位、包封特性、FESEM、ATR-FTIR、XRD、DSC、体外释放、抗氧化和抗增殖特性分析。纳米胶囊（粒径 119.50-365.21nm）呈球形至不规则形状，ζ-电位为正（17.50-18.09mV）。5mg/g HT（HTS1）和 20mg/g HT（HTS2）的包封效率分别为 77.13%和 56.30%。纳米胶囊为无定形，结晶度为 12.34%-15.48%，结晶度为 20nm-27nm。纳米胶囊的形成导致玻璃化转变温度升高。在模拟胃肠道消化结束时，HTS2 释放了 67.12%的 HT（HTS1 释放了 58.89%）。与游离 HT 相比，纳米包封的 HT 具有更高的抗氧化和抗增殖（对 A549 和 MDA-MB-231 癌细胞系）特性。