Sy Kadiatou, Agossa Kevimy, Maton Mickaël, Chijcheapaza-Flores Henry, Martel Bernard, Siepmann Florence, Deveaux Etienne, Blanchemain Nicolas, Neut Christel
U1008-Controlled Drug Delivery Systems and Biomaterials, Inserm, CHU Lille, University of Lille, 59000 Lille, France.
UMR 8207, UMET-Unité Matériaux et Transformations, CNRS-Centre National de la Recherche Scientifique, INRA-Institut National de la Recherche Agronomique, ENSCL-Ecole Nationale Supérieure de Chimie de Lille, University of Lille, 59655 Lille, France.
Antibiotics (Basel). 2021 Nov 5;10(11):1352. doi: 10.3390/antibiotics10111352.
The aim of our study was to explore the potential value of metallic (Ag, Cu, and Zn) salts, polymer/metallic nanoparticles, and chlorhexidine (CHX) for improving the antimicrobial activity of calcium hydroxide (CH) against and , associated with persistent endodontic infections. A first screening was performed by determining minimum inhibitory/bactericidal concentrations (MIC/MBC). Antimicrobial activity of the CH paste mixed with metallic salts, chitosan or cyclodextrin polymer metallic nanoparticles was compared to the antimicrobial activity of CH paste alone and CH + CHX using a time-kill kinetics assay. The effect of the antimicrobials on the rheological and the key mechanical properties were also examined. Copper and zinc were discarded because of their MIC/MBC values and silver because of its kill time curve profile. Except for a slower setting time after 24 h and a higher weight loss after 1 week of incubation, the mechanical behavior of the CH paste was unaffected by the addition of CHX. Polymeric/metallic nanoparticles failed to potentiate the antimicrobial effect of CH. By contrast, CHX increased this effect and thus could help eradicate associated with persistent root canal infections without altering the desired key physical properties of the CH paste.
我们研究的目的是探索金属（银、铜和锌）盐、聚合物/金属纳米颗粒以及洗必泰（CHX）对于提高氢氧化钙（CH）对与持续性牙髓感染相关的[具体细菌名称1]和[具体细菌名称2]的抗菌活性的潜在价值。通过测定最低抑菌/杀菌浓度（MIC/MBC）进行初步筛选。使用时间-杀菌动力学分析法，将与金属盐、壳聚糖或环糊精聚合物金属纳米颗粒混合的CH糊剂的抗菌活性与单独的CH糊剂以及CH + CHX的抗菌活性进行比较。还研究了抗菌剂对流变学和关键力学性能的影响。由于铜和锌的MIC/MBC值而将其排除，银则因其杀菌时间曲线特征而被排除。除了24小时后凝固时间延长以及孵育1周后重量损失增加外，添加CHX对CH糊剂的力学性能没有影响。聚合物/金属纳米颗粒未能增强CH的抗菌效果。相比之下，CHX增强了这种效果，因此有助于根除与持续性根管感染相关的[具体细菌名称1]和[具体细菌名称2]，同时不会改变CH糊剂所需的关键物理性能。
