Key Laboratory of Modern Acoustics (MOE), Department of Physics, Collaborative Innovation Center of Advanced Microstructure, Nanjing University, Nanjing 210093, China.
Key Laboratory of Modern Acoustics (MOE), Department of Physics, Collaborative Innovation Center of Advanced Microstructure, Nanjing University, Nanjing 210093, China.
Ultrason Sonochem. 2017 Nov;39:863-871. doi: 10.1016/j.ultsonch.2017.06.016. Epub 2017 Jun 20.
Sonoporation has been widely accepted as a significant tool for gene delivery as well as some bio-effects like hemolysis, bringing in high demands of looking into its underlying mechanism. A two-dimensional (2D) boundary element method (BEM) model was developed to investigate microbubble-cell interaction, especially the morphological and mechanical characteristics around the close-to-bubble point (CP) on cell membrane. Based on time evolution analysis of sonoporation, detailed information was extracted from the model for analysis, including volume expansion ratio of the bubble, areal expansion ratio of the cell, jet velocity and CP displacement. Parametric studies were carried out, revealing the influence of different ultrasound parameters (i.e., driving frequency and acoustic pressure) and geometrical configurations (i.e., bubble-cell distance and initial bubble radius). This model could become a powerful tool not only for understanding bubble-cell interactions, but also for optimizing the strategy of sonoporation, such that it could be safer and of higher efficiency for biological and medical studies especially in clinics.
声致孔作用已被广泛认可为一种重要的基因传递工具，以及一些生物效应，如溶血，这就需要深入研究其潜在的机制。本文建立了一个二维（2D）边界元法（BEM）模型，用于研究微泡-细胞相互作用，特别是细胞膜上接近气泡点（CP）处的形态和力学特性。基于声致孔的时变分析，从模型中提取了详细信息进行分析，包括气泡的体积膨胀比、细胞的面积膨胀比、射流速度和 CP 位移。进行了参数研究，揭示了不同超声参数（即驱动频率和声压）和几何结构（即气泡-细胞距离和初始气泡半径）的影响。该模型不仅可以成为理解气泡-细胞相互作用的有力工具，还可以优化声致孔的策略，使其在生物和医学研究中更安全、更高效，特别是在临床中。
