Kovacs J A, Gill V, Swan J C, Ognibene F, Shelhamer J, Parrillo J E, Masur H
Lancet. 1986 Jul 5;2(8497):1-3. doi: 10.1016/s0140-6736(86)92555-9.
To evaluate the ability of a mouse monoclonal antibody, 2G2, directed against human Pneumocystis carinii, to detect the organism in clinical specimens, a prospective study of the antibody in an indirect immunofluorescent assay was undertaken. P carinii was rapidly detected in thirteen of fourteen bronchoalveolar lavage specimens positive by toluidine-blue-O stain, none of eleven lavage specimens negative by toluidine-blue-O, neither of two impression smears of histologically negative open-lung biopsy specimens, and both of two impression smears of histologically positive necropsy specimens. Immunofluorescence with monoclonal antibody 2G2 is a rapid, simple, and specific technique for detection of P carinii in clinical specimens.
为评估一种针对人卡氏肺孢子虫的小鼠单克隆抗体2G2在临床标本中检测该病原体的能力，我们对该抗体进行了间接免疫荧光试验的前瞻性研究。在14份经甲苯胺蓝O染色呈阳性的支气管肺泡灌洗标本中，有13份快速检测到卡氏肺孢子虫；在11份经甲苯胺蓝O染色呈阴性的灌洗标本中均未检测到；在2份组织学检查为阴性的开胸肺活检标本印片中均未检测到；而在2份组织学检查为阳性的尸检标本印片中均检测到。用单克隆抗体2G2进行免疫荧光检测是一种在临床标本中快速、简便且特异的检测卡氏肺孢子虫的技术。
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