Aoki Keita, Niki Hironori
Microbial Genetics Laboratory, Genetic Strains Research Center, National Institute of Genetics, Mishima, Shizuoka 411-8540 Japan.
Microbial Genetics Laboratory, Genetic Strains Research Center, National Institute of Genetics, Mishima, Shizuoka 411-8540 Japan
Cold Spring Harb Protoc. 2017 Dec 1;2017(12):pdb.prot091850. doi: 10.1101/pdb.prot091850.
This protocol describes the use of electroporation to transform with plasmids or linear DNA. Plasmids are helpful for the complementation testing of mutations and for the expression of specific genes. Linear DNA fragments integrated into chromosomal DNA by homologous recombination are useful for gene deletion or to fuse a gene with a tag sequence (e.g., encoding a fluorescent protein). To introduce DNA into , electroporation methods are recommended because is sensitive to lithium acetate (LiOAc).
本方案描述了使用电穿孔法用质粒或线性DNA进行转化。质粒有助于突变的互补测试和特定基因的表达。通过同源重组整合到染色体DNA中的线性DNA片段可用于基因缺失或将基因与标签序列（如编码荧光蛋白的序列）融合。由于[具体生物名称]对醋酸锂（LiOAc）敏感，因此推荐使用电穿孔法将DNA导入[具体生物名称]。
