Sahoo Smita, Awasthi Jay Prakash, Sunkar Ramanjulu, Panda Sanjib Kumar
Plant Molecular Biotechnology Laboratory, Department of Life Science and Bioinformatics, Assam University, Silchar, 788011, Assam, India.
Department of Biochemistry and Molecular Biology, Oklahoma State University, Stillwater, OK, USA.
Methods Mol Biol. 2017;1631:273-277. doi: 10.1007/978-1-4939-7136-7_16.
Upon exposure to abiotic stresses, plants tend to accumulate excessive amounts of reactive oxygen species (ROS) that inturn react with cellular lipids, proteins, and DNA. Therefore, decreasing ROS accumulation is indispensible to survive under stress, which is accomplished by inducing enzymatic and nonenzymatic antioxidant defense pathways. Glutathione, particularly reduced glutathione (GSH), represents a principal anitioxidant that could decrease ROS through scavenging them directly or indirectly through ascorbate-glutathione cycle or GSH peroxidases. Glutathione content can be determined using HPLC or spectrophotometric assays. In this chapter, we provided detailed assays to determine total, reduced, and oxidized gluathione using spectrophotometric method.
在遭受非生物胁迫时，植物往往会积累过量的活性氧（ROS），这些活性氧进而会与细胞内的脂质、蛋白质和DNA发生反应。因此，减少ROS的积累对于在胁迫条件下存活至关重要，这是通过诱导酶促和非酶促抗氧化防御途径来实现的。谷胱甘肽，特别是还原型谷胱甘肽（GSH），是一种主要的抗氧化剂，它可以通过直接清除ROS或通过抗坏血酸-谷胱甘肽循环或谷胱甘肽过氧化物酶间接清除ROS来降低ROS水平。谷胱甘肽含量可以使用高效液相色谱法（HPLC）或分光光度法进行测定。在本章中，我们提供了使用分光光度法测定总谷胱甘肽、还原型谷胱甘肽和氧化型谷胱甘肽的详细方法。
