Jones Patricia M, Boriack Richard, Struys Eduard A, Rakheja Dinesh
Department of Pathology, University of Texas Southwestern Medical Center, Dallas, TX, USA.
Department of Pathology and Laboratory Medicine, Children's Health, Children's Medical Center, Dallas, TX, USA.
Methods Mol Biol. 2017;1633:219-234. doi: 10.1007/978-1-4939-7142-8_14.
We describe a liquid chromatography-tandem mass spectrometry assay for measurement of D-2-hydroxyglutaric acid and L-2-hydroxyglutaric acid. These metabolites are increased in specific inborn errors of metabolism and are now recognized as oncometabolites. The measurement of D-2-hydroxyglutarate in peripheral blood may be used as a biomarker for screening and follow-up of patients with IDH-mutated acute myeloid leukemia.
我们描述了一种用于测量D-2-羟基戊二酸和L-2-羟基戊二酸的液相色谱-串联质谱分析法。这些代谢物在特定的先天性代谢缺陷中会增加，现在被认为是肿瘤代谢物。外周血中D-2-羟基戊二酸的测量可用作IDH突变的急性髓系白血病患者筛查和随访的生物标志物。
