Yin Zhao-Yang, Qi Si-Yong, Cheng Xiang, Guo Liang, Chen Hong-Yu, Shi Ming
The Health Team, Shandong Armed Police Corps Detachment of Laiwu City, Laiwu 271199, China.E-mail:
Nan Fang Yi Ke Da Xue Xue Bao. 2017 Jul 20;37(7):914-918. doi: 10.3969/j.issn.1673-4254.2017.07.10.
To explore an efficient and reliable method for establishing an animal model of bone metastasis of tumors.
Male C57BL/6J mice were randomized into 4 equal groups to receive injections of normal saline or prostate cancer RM-1 cell suspension via the femoral artery or the external iliac artery, and breast cancer 4T1-luc cells were injected in 15 female BALB/c mice via the femoral artery. The operation time, postoperative survival rate, and the tumor formation rates in the mice with two different injection methods were compared. The tumor metastasis in the mice was evaluated with in vivo imaging.
The mean time for hemostasis time was 2.53∓1.75 min in mice receiving tumor cell injection via the femoral artery, significantly shorter than that in mice with injections via the external iliac artery (4.70∓1.63 min; P<0.05); the mean operation time was 14.67∓2.16 min and 22.47∓3.50 min in the two groups, respectively (P<0.05). At 21 days after the operation, the survival rate was 93.3% in femoral artery injection group, significantly higher than that in external iliac artery injection group (66.7%;P<0.05). The tumor metastasis rate was 100% in both groups.
Injection of the tumor cells via the femoral artery is more suitable for establishing mouse models of bone metastasis of cancers.
探索一种高效、可靠的建立肿瘤骨转移动物模型的方法。
将雄性C57BL/6J小鼠随机分为4组，每组数量相等，分别经股动脉或髂外动脉注射生理盐水或前列腺癌RM-1细胞悬液，对15只雌性BALB/c小鼠经股动脉注射乳腺癌4T1-luc细胞。比较两种不同注射方法小鼠的手术时间、术后生存率及肿瘤形成率。采用活体成像评估小鼠的肿瘤转移情况。
经股动脉注射肿瘤细胞的小鼠平均止血时间为2.53±1.75分钟，明显短于经髂外动脉注射的小鼠（4.70±1.63分钟；P<0.05）；两组的平均手术时间分别为14.67±2.16分钟和22.47±3.50分钟（P<0.05）。术后21天，股动脉注射组的生存率为93.3%，明显高于髂外动脉注射组（66.7%；P<0.05）。两组的肿瘤转移率均为100%。
经股动脉注射肿瘤细胞更适合建立癌症骨转移小鼠模型。
