葡萄糖调节蛋白78参与PCA3调控的前列腺癌进展。
GRP78 Participates in PCA3-regulated Prostate Cancer Progression.
作者信息
Lin Chunhua, Wang Jiahui, Wang Yanwei, Zhu Peng, Liu Xin, Li Ning, Liu Juan, Yu Luxin, Wang Wenting
机构信息
Department of Urological Surgery, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, P.R. China.
Central Laboratory, The Affiliated Yantai Yuhuangding Hospital of Qingdao University, Yantai, P.R. China.
出版信息
Anticancer Res. 2017 Aug;37(8):4303-4310. doi: 10.21873/anticanres.11823.
BACKGROUND/AIM: The human prostate cancer antigen 3 (PCA3) is a long non-coding RNA (lncRNA) commonly used as a diagnostic marker for prostate cancer (PCa). Herein we investigated the cellular function of PCA3 in PCa and its potential mechanism.
MATERIALS AND METHODS
PCA3 was overexpressed in a PC3 cell line (PC3) and cell proliferation, migration, invasion and apoptosis were compared to those of control cells (PC3). Differentially expressed proteins were identified by two-dimensional polyacrylamide gel electrophoresis (2D-PAGE) and mass spectrometry.
RESULTS
Overexpression of PCA3 significantly increased cell proliferation rate, migration and invasion, while inhibited apoptosis in PC3 cells. Three proteins were found down-regulated and 7 proteins up-regulated in PC3 cells compared to PC3 cells, including GRP78. Higher GRP78 was also found in PCa clinical specimens.
CONCLUSION
This study confirmed that in PCa, PCA3 plays a pro-cancer role through promoting cell proliferation, migration and invasion while inhibiting cell apoptosis. This process might involve the up-regulation of GRP78.
背景/目的：人前列腺癌抗原3（PCA3）是一种长链非编码RNA（lncRNA），常用于前列腺癌（PCa）的诊断标志物。在此，我们研究了PCA3在PCa中的细胞功能及其潜在机制。
材料与方法
在PC3细胞系（PC3）中过表达PCA3，并将细胞增殖、迁移、侵袭和凋亡与对照细胞（PC3）进行比较。通过二维聚丙烯酰胺凝胶电泳（2D-PAGE）和质谱鉴定差异表达的蛋白质。
结果
PCA3的过表达显著提高了PC3细胞的增殖率、迁移和侵袭能力，同时抑制了细胞凋亡。与PC3细胞相比，在PC3细胞中发现3种蛋白质下调，7种蛋白质上调，包括GRP78。在PCa临床标本中也发现较高水平的GRP78。
结论
本研究证实，在PCa中，PCA3通过促进细胞增殖、迁移和侵袭，同时抑制细胞凋亡发挥促癌作用。这一过程可能涉及GRP78的上调。
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