Guo Suiqun, Xiao Yanyi, Li Danqing, Jiang Qingping, Zhu Litong, Lin Dan, Jiang Huiping, Chen Wei, Wang Lijing, Liu Chunhua, Fang Weiyi, Lin Li
Department of Obstetrics and Gynecology, The Third Affiliated Hospital of Southern Medical University, Guangzhou, 510630, P.R. China.
Department of Healthy Management, Nanfang Hospital, Southern Medical University, Guangzhou, Guangdong, 510515, P.R. China.
Oncotarget. 2017 Dec 7;9(1):680-690. doi: 10.18632/oncotarget.23090. eCollection 2018 Jan 2.
The aim of this study was to measure the expression patterns of PGK1 and GRP78 in normal endometrial tissues and endometrial carcinoma, and associations between their combined effects and the pathological features of endometrial carcinoma. We used 30 normal endometrial tissue samples and 130 endometrial carcinoma samples, and separately evaluated PGK1 and GRP78 protein expression by immunohistochemistry. Scores ranging from 0 to 9 were obtained by multiplying the percentage of positive cells by the staining intensity (0-3). Immunohistochemical analysis revealed increased PGK1 and GRP78 expression in the cytoplasm of endometrial carcinoma cells compared with that in normal endometrial tissues. High PGK1 expression positively correlated with the FIGO stage ( < 0.001), histological grade ( = 0.002), and lymph node status ( < 0.001). High GRP78 expression positively correlated with the pathological type ( = 0.0125), FIGO stage ( < 0.001), and lymph node status ( < 0.001). In addition, PGK1 overexpression was positively correlated with GRP78 overexpression in endometrial carcinoma patients ( < 0.001), and the concurrent expression of both oncogenes in endometrial carcinoma patients correlated significantly with the lymph node status ( < 0.001) and FIGO stage ( < 0.001). Patients with high PGK1 and GRP78 expression levels had poorer overall survival rates than those with low expression levels of both proteins ( < 0.001). Our results suggested that the co-occurrence of PGK1 and GRP78 expression is potentially an unfavorable factor for endometrial carcinoma progression.
本研究旨在检测磷酸甘油酸激酶1（PGK1）和葡萄糖调节蛋白78（GRP78）在正常子宫内膜组织和子宫内膜癌中的表达模式，以及它们的联合作用与子宫内膜癌病理特征之间的关联。我们使用了30例正常子宫内膜组织样本和130例子宫内膜癌样本，并通过免疫组织化学分别评估PGK1和GRP78蛋白表达。通过将阳性细胞百分比乘以染色强度（0 - 3）获得0至9分的评分。免疫组织化学分析显示，与正常子宫内膜组织相比，子宫内膜癌细胞胞质中PGK1和GRP78表达增加。高PGK1表达与国际妇产科联盟（FIGO）分期（<0.001）、组织学分级（=0.002）和淋巴结状态（<0.001）呈正相关。高GRP78表达与病理类型（=0.0125）、FIGO分期（<0.001）和淋巴结状态（<0.001）呈正相关。此外，在子宫内膜癌患者中，PGK1过表达与GRP78过表达呈正相关（<0.001），并且这两种癌基因在子宫内膜癌患者中的同时表达与淋巴结状态（<0.001）和FIGO分期（<0.001）显著相关。PGK1和GRP78表达水平高的患者总生存率低于两种蛋白表达水平低的患者（<0.001）。我们的结果表明，PGK1和GRP78表达同时出现可能是子宫内膜癌进展的不利因素。
